5 Monivong Boulevard, P.O Box. 983, Phnom Penh, Cambodia accueil@pasteur-kh.org

The laboratory of Microbiology, managed by HEM Sopheak under the supervision of Alexandra KERLEGUER, is a part of Medical Laboratory Unit which has three disciplines: Bacteriology, Parasitology and Mycology. This laboratory is one of the major laboratories in Cambodia, and it conducts research on bacteria, parasites and fungal agents from human infections.

Main objective is to follow antibiotic susceptibility. Besides providing routine analysis for individual patients, public and private hospitals and NGOs, the laboratory also participates in many collaborative research projects with the research units of the Institut Pasteur du Cambodge, the Institut Pasteur International Network (IPIN), national or international NGOs and public health organizations.

Previous collaborative research activities have been conducted on H1N1 pandemic virus infection in hospital, Cambodia (IMMI Project); Community­ acquired acute lower respiratory infection among hospitalized patients, in Takeo and Kampong Cham provinces, 2007 – 2011 (SISEA project); Phylogeography of Shigella spp in Southeast Asia; Burden of Leptospirosis in Cambodia; SEAe: Infections of the central nervous system –Meningoencephalitis; surveillance of antimicrobial resistance in Salmonella spp, Campylobacter spp, E. coli and Enterococcus spp from poultry carcasses and Salmonella spp and Campylobacter spp (AGISAR)

Current collaborative research activities are conducted on Antibiotic Resistant Infections in Children in Low Income Countries (Birdy project); Klebsiella pneumoniae, and Aspergillus and Aspergillosis in Cambodia.

Staff: CHHEANG Rattanak, NOP Puthea, MENG Soda, PRAK Sivorn, KHUON Makara, NOR Vuthea

 

Antimicrobial Resistance Research Group

Although antibiotics are not usually used appropriately, few data published on the antimicrobial resistance in Cambodia. Epidemic dissemination and the transfer of the genes of resistance among other bacterial species put the population at risk. Response to this issue, our group project aim is to:

  • Assess the frequency of Enterobacteria producing ESBL and carbapenemase,
  • Estimate the fecal carriage rate,
  • Study the characterization of resistance gene by molecular technique,
  • Assess the repartition rate of hospitalized and community infections by sequencing.

This group is leaded by Alexandra KERLEGUER, MD, Medical Biologist.

Scientific collaborators: HEM Sopheak, Pharm. D, Medical Biologist; CHEANG Rattanak, MD; HENG Seiha, Engineer Molecular Biology; NOP Puthea, Laboratory Technologist

 

Laboratory of Mycobacteriology

The Laboratory of Mycobacteriology, which is part of the Medical Laboratory Unit, is the only center with BSL-3 facility for mycobacteria testing, and molecular biology testing in Cambodia. We provide laboratory service for TB and drug resistance TB diagnosis to the public and private hospitals/organizations. Beside routine laboratory activities, the laboratory has conducted and participated in many research studies on tuberculosis and non-tuberculous mycobacteria by cooperating with the National Tuberculosis Control Program (NTP) and local and international NGOs.

Previous research activities:

We participated with the NTP to the first national drug resistance surveillance in 2000 and the first national TB prevalence survey in 2002 in Cambodia. Since then, the laboratory has been involved in prevalence studies (MTB disease in children and in adult co-infected with HIV), diagnosis studies (evaluated a new tool and molecular marker for tuberculosis diagnosis and an algorithm for improving diagnosis of TB in HIV co-infected children and adult), clinical trials (CAMELIA) and molecular epidemiology and drug resistance studies on MTB. Since 2012, the laboratory has been a member of the international young team (MySA) within Southeast Asia region involving Vietnam, Lao PDR and Cambodia to conduct molecular epidemiology and drug resistance study of MTB at the regional level supported by the Institute of Research for Development (IRD).

The Laboratory of Mycobacteriology is managed by CHENG Sokleaph under supervision of Alexandra KERLÉGUER.

Staff: HIM Vutheavy, SRENG Narom and KEO Sophany

The laboratory is currently implicating in several research project:

Project of International Joint Laboratory (LMI) “Drug Resistance in Southeast Asia”: The aim of this LMI is to get insight into the mechanisms of resistance emergence and transmission at various spatial and temporal scales. This study involves three countries, Vietnam, Laos and Cambodia.

Sequence and Analysis of Non-tuberculous Mycobacteria (NTM) strains: The objective of the study is to characterize the NTM strains representing geographically diverse TB-endemic areas with high NTM prevalence using the NGS method. The study was funded by the National Institute of Health. Co-PI: CHENG Sokleaph.

Genetic determinants and evolution of drug resistance in Mycobacterium tuberculosis in Cambodia: We aim to characterize genetic determinants and to study the evolution of drug resistance in MTB in Cambodia. PI: CHENG Sokleaph.