5 Monivong Boulevard, P.O Box. 983, Phnom Penh, Cambodia [email protected]

The medical entomology platform has been recently created at IPC, in 2015. This PF includes an insectary for rearing mosquito colonies (Anopheles, Culex and Aedes). A collection room for training and reference specimens is under construction.

The medical entomology platform is managed by Amélie VANTAUX (Malaria Molecular Epidemiology unit) and DUONG Veasna (Virology unit) under supervision of Didier FONTENILLE.

Staff is: PENG Borin (Virology), NEUNG Chhoueth (Virology), PANG Senglong (Virology), YEAN Sony (Virology), SAMRETH Reingsey (Malaria Molecular Epidemiology), EAK Por Piv (Malaria Molecular Epidemiology)

The platform is implicated in several projects from Malaria, Virology and Epidemiology units:

Malaria: Role of vectorial transmission on the evolution of Plasmodium falciparum artemisinin resistance: using in vitro gametocyte culture we will assess the infectivity of artemisinin-resistant and artemisinin-susceptible strains to the mosquito vector. PI: Amélie VANTAUX, Malaria Molecular Epidemiology Unit.

Lacanet project: The vector component of this project is to sample, to identify and to test for arboviruses, wild mosquitoes collected in Mondulkiri and Kompong Thom provinces. PI: Paul HORWOOD, Virology unit

Japanese Encephalitis (JE): 2 projects aim to document the burden and the transmission of JE virus SEAe projects PI Philippe DUSSART, Virology unit, and Comaccross project PI Julien CAPPELLE, epidemiology unit. Diversity, dynamics and implication in JEV transmission of potential vectors (mainly Culex) are assessed within the Comaccross projects, in Kandal and Ta Khmau.

Zika Acip project: Study of vector competence and vertical transmission of ZIKAV in Cambodia Aedes spp. Mosquitoes. Co-PI: Veasna DUONG, Virology unit.

Chikungunya: WP2 of a PTR project. Evolution of chikungunya virus Caribbean strains in American and Asian mosquitoes. The objective of this WP is to identify naturally emerging variants during persistent infection of mosquitoes and predict the evolutionary trajectory of the Caribbean strain during natural transmission cycles. Since this strain first originated in Asia, its evolution in Asian mosquitoes will also be determined for comparison. Co-PI: Veasna DUONG, Virology unit.

Project finishing in 2016:
Ecomore project: WP1 (PI Arnaud Tarantola): Improving the monitoring of disease emergence (using Chikungunya and Dengue as proxies) and progression related to changes in human demographics and transportation.
Entomological activities are related to the development of an early warning tool by testing a simple entomological tool for assessing variation in mosquito “bio-mass” (including Aedes mosquitoes), combined with meteorological data.

Several other projects on mosquito biology and control are under discussion with Cambodian partners (mainly CNM) and International partners (IP, IRD, Cirad, CNRS, malaria consortium, US CDC, …).