5 Monivong Boulevard, P.O Box. 983, Phnom Penh, Cambodia [email protected]

I have joined the Malaria Molecular Epidemiology Unit in Institut Pasteur in Cambodia as engineer since 2001. I am a researcher and in charge of Molecular Biology Platform in this Unit after I got my PhD from University of Montpellier in 2014. Our activities focus on improving tools to detect malaria asexual and sexual Plasmodium stages in asymptomatic parasite carriers in order to evaluate the impact of the strategies implemented by National Center for Parasitology, Entomology and Malaria control in Cambodia. I am also working on the development of molecular tools to assess the antimalarial drug resistance of Plasmodium falciparum.

Publications in 2017

  • Witkowski B, Duru V, Khim N, Ross LS, Saintpierre B, Beghain J, Chy S, Kim S, Ke S, Kloeung N, Eam R, Khean C, Ken M, Loch K, Bouillon A, Domergue A, Ma L, Bouchier C, Leang R, Huy R, Nuel G, Barale JC, Legrand E, Ringwald P, Fidock DA, Mercereau-Puijalon O, Ariey F, Menard D. A surrogate marker of piperaquine-resistant Plasmodium falciparum malaria: a phenotype-genotype association study. Lancet Infect Dis. 2017;17(2):174-183.
  • Rossi G, Van den Bergh R, Nguon C, Debackere M, Vernaeve L, Khim N, Kim S, Menard D, De Smet M, Kindermans JM. Adapting reactive case detection strategies for falciparum malaria in a low-transmission area in Cambodia. Clin Infect Dis.
  • Rossi G, De Smet M, Khim N, Kindermans JM, Menard D. Performance of Rapid Diagnostic Testing in Patients with Suspected Malaria in Cambodia, a Low-Endemicity Country Aiming for Malaria Elimination. Clin Infect Dis. 2017;65(10):1769-1770.
  • Rossi G, De Smet M, Khim N, Kindermans JM, Menard D. Emergence of Plasmodium falciparum triple mutant in Cambodia. Lancet Infect Dis. 2017;17(12):1233.

 

Publications in 2016

  • Witkowski B, Khim N, Kim S, Domergue A, Duru V, Menard D. [Multiple and successive treatment failures in a patient infected by Plasmodium falciparum in Cambodia and treated by dihydroartemisinin-piperaquine]. Bull Soc Pathol Exot. 2016;109(2):87-90.
  • Sluydts V, Durnez L, Heng S, Gryseels C, Canier L, Kim S, Van Roey K, Kerkhof K, Khim N, Mao S, Uk S, Sovannaroth S, Grietens KP, Sochantha T, Menard D, Coosemans M. Efficacy of topical mosquito repellent (picaridin) plus long-lasting insecticidal nets versus long-lasting insecticidal nets alone for control of malaria: a cluster randomised controlled trial. Lancet Infect Dis. 2016;16(10):1169-1177.
  • Menard D, Khim N, Beghain J, Adegnika AA, Shafiul-Alam M, Amodu O, Rahim-Awab G, Barnadas C, Berry A, Boum Y, Bustos MD, Cao J, Chen JH, Collet L, Cui L, Thakur GD, Dieye A, Djalle D, Dorkenoo MA, Eboumbou-Moukoko CE, Espino FE, Fandeur T, Ferreira-da-Cruz MF, Fola AA, Fuehrer HP, Hassan AM, Herrera S, Hongvanthong B, Houze S, Ibrahim ML, Jahirul-Karim M, Jiang L, Kano S, Ali-Khan W, Khanthavong M, Kremsner PG, Lacerda M, Leang R, Leelawong M, Li M, Lin K, Mazarati JB, Menard S, Morlais I, Muhindo-Mavoko H, Musset L, Na-Bangchang K, Nambozi M, Niare K, Noedl H, Ouedraogo JB, Pillai DR, Pradines B, Quang-Phuc B, Ramharter M, Randrianarivelojosia M, Sattabongkot J, Sheikh-Omar A, Silue KD, Sirima SB, Sutherland C, Syafruddin D, Tahar R, Tang LH, Toure OA, Tshibangu-wa-Tshibangu P, Vigan-Womas I, Warsame M, Wini L, Zakeri S, Kim S, Eam R, Berne L, Khean C, Chy S, Ken M, Loch K, Canier L, Duru V, Legrand E, Barale JC, Stokes B, Straimer J, Witkowski B, Fidock DA, Rogier C, Ringwald P, Ariey F, Mercereau-Puijalon O, Consortium K. A Worldwide Map of Plasmodium falciparum K13-Propeller Polymorphisms. N Engl J Med. 2016;374(25):2453-2464.
  • Leang R, Canavati SE, Khim N, Vestergaard LS, Borghini Fuhrer I, Kim S, Denis MB, Heng P, Tol B, Huy R, Duparc S, Dondorp AM, Menard D, Ringwald P. Efficacy and Safety of Pyronaridine-Artesunate for Treatment of Uncomplicated Plasmodium falciparum Malaria in Western Cambodia. Antimicrob Agents Chemother. 2016;60(7):3884-3890.
  • Hustedt J, Canavati SE, Rang C, Ashton RA, Khim N, Berne L, Kim S, Sovannaroth S, Ly P, Menard D, Cox J, Meek S, Roca-Feltrer A. Reactive case-detection of malaria in Pailin Province, Western Cambodia: lessons from a year-long evaluation in a pre-elimination setting. Malar J. 2016;15:132.
  • Dwivedi A, Khim N, Reynes C, Ravel P, Ma L, Tichit M, Bourchier C, Kim S, Dourng D, Khean C, Chim P, Siv S, Frutos R, Lek D, Mercereau-Puijalon O, Ariey F, Menard D, Cornillot E. Plasmodium falciparum parasite population structure and gene flow associated to anti-malarial drugs resistance in Cambodia. Malar J. 2016;15:319.
  • Beghain J, Langlois AC, Legrand E, Grange L, Khim N, Witkowski B, Duru V, Ma L, Bouchier C, Menard D, Paul RE, Ariey F. Plasmodium copy number variation scan: gene copy numbers evaluation in haploid genomes. Malar J. 2016;15:206.

 

Publications in 2015

  • Straimer J, Gnadig NF, Witkowski B, Amaratunga C, Duru V, Ramadani AP, Dacheux M, Khim N, Zhang L, Lam S, Gregory PD, Urnov FD, Mercereau-Puijalon O, Benoit-Vical F, Fairhurst RM, Menard D, Fidock DA. Drug resistance. K13-propeller mutations confer artemisinin resistance in Plasmodium falciparum clinical isolates. 2015;347(6220):428-431.
  • Leang R, Taylor WR, Bouth DM, Song L, Tarning J, Char MC, Kim S, Witkowski B, Duru V, Domergue A, Khim N, Ringwald P, Menard D. Evidence of Plasmodium falciparum Malaria Multidrug Resistance to Artemisinin and Piperaquine in Western Cambodia: Dihydroartemisinin-Piperaquine Open-Label Multicenter Clinical Assessment. Antimicrob Agents Chemother. 2015;59(8):4719-4726.
  • Edwards HM, Canavati SE, Rang C, Ly P, Sovannaroth S, Canier L, Khim N, Menard D, Ashton RA, Meek SR, Roca-Feltrer A. Novel Cross-Border Approaches to Optimise Identification of Asymptomatic and Artemisinin-Resistant Plasmodium Infection in Mobile Populations Crossing Cambodian Borders. PLoS One. 2015;10(9):e0124300.
  • Duru V, Khim N, Leang R, Kim S, Domergue A, Kloeung N, Ke S, Chy S, Eam R, Khean C, Loch K, Ken M, Lek D, Beghain J, Ariey F, Guerin PJ, Huy R, Mercereau-Puijalon O, Witkowski B, Menard D. Plasmodium falciparum dihydroartemisinin-piperaquine failures in Cambodia are associated with mutant K13 parasites presenting high survival rates in novel piperaquine in vitro assays: retrospective and prospective investigations. BMC Med. 2015;13:305.
  • Canier L, Khim N, Kim S, Eam R, Khean C, Loch K, Ken M, Pannus P, Bosman P, Stassijns J, Nackers F, Alipon S, Char MC, Chea N, Etienne W, De Smet M, Kindermans JM, Menard D. Malaria PCR detection in Cambodian low-transmission settings: dried blood spots versus venous blood samples. Am J Trop Med Hyg. 2015;92(3):573-577.

 

Publications in 2014

  • Sluydts V, Heng S, Coosemans M, Van Roey K, Gryseels C, Canier L, Kim S, Khim N, Siv S, Mean V, Uk S, Grietens KP, Tho S, Menard D, Durnez L. Spatial clustering and risk factors of malaria infections in Ratanakiri Province, Cambodia. Malar J. 2014;13:387.
  • Roca-Feltrer A, Khim N, Kim S, Chy S, Canier L, Kerleguer A, Tor P, Chuor CM, Kheng S, Siv S, Kachur PS, Taylor WR, Hwang J, Menard D. Field trial evaluation of the performances of point-of-care tests for screening G6PD deficiency in Cambodia. PLoS One. 2014;9(12):e116143.
  • Mueller TC, Siv S, Khim N, Kim S, Fleischmann E, Ariey F, Buchy P, Guillard B, Gonzalez IJ, Christophel EM, Abdur R, von Sonnenburg F, Bell D, Menard D. Acute undifferentiated febrile illness in rural Cambodia: a 3-year prospective observational study. PLoS One. 2014;9(4):e95868.
  • Khim N, Witkowski B, Duru V, Ariey F, Mercereau-Puijalon O, Menard D. [Towards a better understanding of the molecular mechanisms of P. falciparum resistance to artemisinin]. Med Sci (Paris). 2014;30(8-9):727-729.
  • Khim N, Andrianaranjaka V, Popovici J, Kim S, Ratsimbasoa A, Benedet C, Barnadas C, Durand R, Thellier M, Legrand E, Musset L, Menegon M, Severini C, Nour BY, Tichit M, Bouchier C, Mercereau-Puijalon O, Menard D. Effects of mefloquine use on Plasmodium vivax multidrug resistance. Emerg Infect Dis. 2014;20(10):1637-1644.
  • Ariey F, Witkowski B, Amaratunga C, Beghain J, Langlois AC, Khim N, Kim S, Duru V, Bouchier C, Ma L, Lim P, Leang R, Duong S, Sreng S, Suon S, Chuor CM, Bout DM, Menard S, Rogers WO, Genton B, Fandeur T, Miotto O, Ringwald P, Le Bras J, Berry A, Barale JC, Fairhurst RM, Benoit-Vical F, Mercereau-Puijalon O, Menard D. A molecular marker of artemisinin-resistant Plasmodium falciparum malaria. 2014;505(7481):50-55.
  • Amaratunga C, Witkowski B, Khim N, Menard D, Fairhurst RM. Artemisinin resistance in Plasmodium falciparum. Lancet Infect Dis. 2014;14(6):449-450.
  • Amaratunga C, Witkowski B, Dek D, Try V, Khim N, Miotto O, Menard D, Fairhurst RM. Plasmodium falciparum founder populations in western Cambodia have reduced artemisinin sensitivity in vitro. Antimicrob Agents Chemother. 2014;58(8):4935-4937.

 

Publications in 2013

  • Witkowski B, Khim N, Chim P, Kim S, Ke S, Kloeung N, Chy S, Duong S, Leang R, Ringwald P, Dondorp AM, Tripura R, Benoit-Vical F, Berry A, Gorgette O, Ariey F, Barale JC, Mercereau-Puijalon O, Menard D. Reduced artemisinin susceptibility of Plasmodium falciparum ring stages in western Cambodia. Antimicrob Agents Chemother. 2013;57(2):914-923.
  • Witkowski B, Amaratunga C, Khim N, Sreng S, Chim P, Kim S, Lim P, Mao S, Sopha C, Sam B, Anderson JM, Duong S, Chuor CM, Taylor WR, Suon S, Mercereau-Puijalon O, Fairhurst RM, Menard D. Novel phenotypic assays for the detection of artemisinin-resistant Plasmodium falciparum malaria in Cambodia: in-vitro and ex-vivo drug-response studies. Lancet Infect Dis. 2013;13(12):1043-1049.
  • Menard D, Andriantsoanirina V, Khim N, Ratsimbasoa A, Witkowski B, Benedet C, Canier L, Mercereau-Puijalon O, Durand R. Global analysis of Plasmodium falciparum Na(+)/H(+) exchanger (pfnhe-1) allele polymorphism and its usefulness as a marker of in vitro resistance to quinine. Int J Parasitol Drugs Drug Resist. 2013;3:8-19.
  • Khim N, Benedet C, Kim S, Kheng S, Siv S, Leang R, Lek S, Muth S, Chea N, Chuor CM, Duong S, Kerleguer A, Tor P, Chim P, Canier L, Witkowski B, Taylor WR, Menard D. G6PD deficiency in Plasmodium falciparum and Plasmodium vivax malaria-infected Cambodian patients. Malar J. 2013;12:171.
  • Canier L, Khim N, Kim S, Sluydts V, Heng S, Dourng D, Eam R, Chy S, Khean C, Loch K, Ken M, Lim H, Siv S, Tho S, Masse-Navette P, Gryseels C, Uk S, Van Roey K, Grietens KP, Sokny M, Thavrin B, Chuor CM, Deubel V, Durnez L, Coosemans M, Menard D. An innovative tool for moving malaria PCR detection of parasite reservoir into the field. Malar J. 2013;12:405.
  • Andriantsoanirina V, Khim N, Ratsimbasoa A, Witkowski B, Benedet C, Canier L, Bouchier C, Tichit M, Durand R, Menard D. Plasmodium falciparum Na+/H+ exchanger (pfnhe-1) genetic polymorphism in Indian Ocean malaria-endemic areas. Am J Trop Med Hyg. 2013;88(1):37-42.

 

Previous Publications

  • Khim N, Kim S, Bouchier C, Tichit M, Ariey F, Fandeur T, Chim P, Ke S, Sum S, Man S, Ratsimbasoa A, Durand R, Menard D. Reduced impact of pyrimethamine drug pressure on Plasmodium malariae dihydrofolate reductase gene. Antimicrob Agents Chemother. 2012;56(2):863-868.
  • Hoyer S, Nguon S, Kim S, Habib N, Khim N, Sum S, Christophel EM, Bjorge S, Thomson A, Kheng S, Chea N, Yok S, Top S, Ros S, Sophal U, Thompson MM, Mellor S, Ariey F, Witkowski B, Yeang C, Yeung S, Duong S, Newman RD, Menard D. Focused Screening and Treatment (FSAT): a PCR-based strategy to detect malaria parasite carriers and contain drug resistant P. falciparum, Pailin, Cambodia. PLoS One. 2012;7(10):e45797.
  • Chou M, Kim S, Khim N, Chy S, Sum S, Dourng D, Canier L, Nguon C, Menard D. Performance of “VIKIA Malaria Ag Pf/Pan” (IMACCESS(R)), a new malaria rapid diagnostic test for detection of symptomatic malaria infections. Malar J. 2012;11:295.
  • Khim N, Siv S, Kim S, Mueller T, Fleischmann E, Singh B, Divis PC, Steenkeste N, Duval L, Bouchier C, Duong S, Ariey F, Menard D. Plasmodium knowlesi infection in humans, Cambodia, 2007-2010. Emerg Infect Dis. 2011;17(10):1900-1902.
  • Lim P, Wongsrichanalai C, Chim P, Khim N, Kim S, Chy S, Sem R, Nhem S, Yi P, Duong S, Bouth DM, Genton B, Beck HP, Gobert JG, Rogers WO, Coppee JY, Fandeur T, Mercereau-Puijalon O, Ringwald P, Le Bras J, Ariey F. Decreased in vitro susceptibility of Plasmodium falciparum isolates to artesunate, mefloquine, chloroquine, and quinine in Cambodia from 2001 to 2007. Antimicrob Agents Chemother. 2010;54(5):2135-2142.
  • Steenkeste N, Dillies MA, Khim N, Sismeiro O, Chy S, Lim P, Crameri A, Bouchier C, Mercereau-Puijalon O, Beck HP, Imwong M, Dondorp AM, Socheat D, Rogier C, Coppee JY, Ariey F. FlexiChip package: an universal microarray with a dedicated analysis software for high-thoughput SNPs detection linked to anti-malarial drug resistance. Malar J. 2009;8:229.
  • Lim P, Alker AP, Khim N, Shah NK, Incardona S, Doung S, Yi P, Bouth DM, Bouchier C, Puijalon OM, Meshnick SR, Wongsrichanalai C, Fandeur T, Le Bras J, Ringwald P, Ariey F. Pfmdr1 copy number and arteminisin derivatives combination therapy failure in falciparum malaria in Cambodia. Malar J. 2009;8:11.
  • Ibrahim ML, Steenkeste N, Khim N, Adam HH, Konate L, Coppee JY, Ariey F, Duchemin JB. Field-based evidence of fast and global increase of Plasmodium falciparum drug-resistance by DNA-microarrays and PCR/RFLP in Niger. Malar J. 2009;8:32.
  • Ibrahim ML, Khim N, Adam HH, Ariey F, Duchemin JB. Polymorphism of PfATPase in Niger: detection of three new point mutations. Malar J. 2009;8:28.
  • Incardona S, Vong S, Chiv L, Lim P, Nhem S, Sem R, Khim N, Doung S, Mercereau-Puijalon O, Fandeur T. Large-scale malaria survey in Cambodia: novel insights on species distribution and risk factors. Malar J. 2007;6:37.
  • Ekala MT, Khim N, Legrand E, Randrianarivelojosia M, Jambou R, Fandeur T, Menard D, Assi SB, Henry MC, Rogier C, Bouchier C, Mercereau-Puijalon O. Sequence analysis of Plasmodium falciparum cytochrome b in multiple geographic sites. Malar J. 2007;6:164.
  • Khim N, Bouchier C, Ekala MT, Incardona S, Lim P, Legrand E, Jambou R, Doung S, Puijalon OM, Fandeur T. Countrywide survey shows very high prevalence of Plasmodium falciparum multilocus resistance genotypes in Cambodia. Antimicrob Agents Chemother. 2005;49(8):3147-3152.
  • Jambou R, Legrand E, Niang M, Khim N, Lim P, Volney B, Ekala MT, Bouchier C, Esterre P, Fandeur T, Mercereau-Puijalon O. Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6. 2005;366(9501):1960-1963.