5 Monivong Boulevard, P.O Box. 983, Phnom Penh, Cambodia accueil@pasteur-kh.org

The Medical Entomology Platform has been recently created at IPC, in 2015. This platform includes an insectary for rearing mosquito colonies (Anopheles, Culex and Aedes). A collection room for training and reference specimens is in progress.

The Medical Entomology Platform is managed by Sebastien BOYER (Medical Entomology Platform) under supervision of Didier FONTENILLE. The platform welcomes the Malaria Molecular Epidemiology Unit (Amélie VANTAUX) and Virology Unit (Veasna DUONG).

Staff is: Sony YEAN, Kimhuor SUOR, Moeun CHHUM (Medical Entomology), Borin PENG, Chhoueth NEUNG, Senglong PANG (Virology), Reingsey SAMRETH (Malaria Molecular and Eakpor PIV (Malaria Molecular Epidemiology)

The platform is implicated in several projects from Malaria, Virology and Epidemiology Units:

Malaria: Role of vectorial transmission on the evolution of Plasmodium falciparum artemisinin resistance: using in vitro gametocyte culture we will assess the infectivity of artemisinin-resistant and artemisinin-susceptible strains to the mosquito vector. PI: Amélie VANTAUX, Malaria Molecular Epidemiology Unit.

Lacanet project: The vector component of this project is to sample, to identify and to test for arboviruses, wild mosquitoes collected in Mondulkiri and Kompong Thom provinces. PI: Philippe DUSSART, Virology Unit

Japanese Encephalitis (JE): 2 projects aim to document the burden and the transmission of JE virus (SEAe projects, PI: Philippe DUSSART, Virology Unit, and ComAcross project, PI: Véronique CHEVALIER, Epidemiology & Public Health Unit). Diversity, dynamics and implication in JEV transmission of potential vectors (mainly Culex) are assessed within the ComAcross projects, in Kandal and Ta Khmau.

Zika Acip project: Study of vector competence and vertical transmission of ZIKAV in Cambodia Aedes spp. Mosquitoes. Co-PI: Veasna DUONG, Virology Unit.

Zikalliance: A Global Alliance for Zika Virus Control and Prevention. Belonging to an international consortium regrouping 53 partners, the project has 3 main objectives:

  • To determine the impact of Zika virus infection during pregnancy and short & medium term effects on newborns,
  • To retrace the natural history of Zika virus infection in humans and their environment in the context of other circulating arboviruses,
  • To built an overall capacity for preparedness research for future epidemic threats.

PI: Xavier DE LAMBALLERIE

Dengue: Ecomore 2 project, financed by AFD, aims to decode the mains anthropogenic and/or ecological mechanisms responsible for the emergence of infectious diseases. The finality of the project is to propose applicable intervention strategies. The project will last until 2018. PI: Sowath LY

Dengue: The Medical Entomology Platform in collaboration with Malaria Consortium study the insecticide resistance status of the main Dengue virus vector, Aedes aegypti. The actual tested insecticides are temephos, permethrin and Bacillus thuringiensis israelensis. Other control methods will be studied. PI: Sebastien BOYER

Chikungunya: WP2 of a PTR project. Evolution of chikungunya virus Caribbean strains in American and Asian mosquitoes. The objective of this WP is to identify naturally emerging variants during persistent infection of mosquitoes and predict the evolutionary trajectory of the Caribbean strain during natural transmission cycles. Since this strain first originated in Asia, its evolution in Asian mosquitoes will also be determined for comparison. Co-PI: Veasna DUONG, Virology Unit.

Project finishing in 2016:

Ecomore project: WP1 (PI Arnaud Tarantola): Improving the monitoring of disease emergence (using Chikungunya and Dengue as proxies) and progression related to changes in human demographics and transportation.

Entomological activities are related to the development of an early warning tool by testing a simple entomological tool for assessing variation in mosquito “bio-mass” (including Aedes mosquitoes), combined with meteorological data.

Several other projects on mosquito biology and control are under discussion with Cambodian partners (mainly CNM) and International partners (IP, IRD, Cirad, CNRS, Malaria Consortium, US CDC, …).

Training

The platform also organizes skilled medical entomology training to develop specific and useful expertise in the country

2016: WHO methods to study Insecticide Resistance (Financial: Malaria Consortium)

2017: Mosquito Taxonomic and Identification Training Course