Seminar on Antimicrobial Resistance of human bacterial strains (excluded TB) isolated in Medical Laboratory
The Committee of Scientific Animation of Institut Pasteur du Cambodge ([email protected]
Thursday 28th of April 2016
At 14h00 in Administration room of Institut Pasteur du Cambodge, 1st floor
Antimicrobial Resistance of human bacterial strains (excluded TB) isolated in Medical Laboratory
by Sopheak Hem, PharmD.
Medical Laboratory Unit, Institut Pasteur in Cambodia
Although antibiotics are not usually used appropriately, few data have been published on the antimicrobial resistance in Cambodia. Epidemic dissemination and the transfer of the genes of resistance among other bacterial species put the population at risk. The aim of this presentation is to determine the frequency and the characteristics of main multidrug resistant bacteria pathogenic such as: extended spectrum beta-lactamase Enterobacteriaceae (ESBLE), bacteria producing carbapenemase, Methicillin-resistant Staphylococcus aureus (MRSA) isolated at Institut Pasteur du Cambodge (IPC) during a four-year period (2012-2015).
Antimicrobial susceptibility testing (AST) was done by disk diffusion on Agar technique, according to the European /French Society for Microbiology (EUCAST/CA-SFM) guidelines.
All AST were automatically processed by using OSIRIS system. E-test was used to determine Minimal Concentration Inhibition in some resistance phenotypes. The analysis of strains shows that the multidrug resistance remains highly strong and are sometimes resistant to all of the antibiotics available in Cambodia. The frequency of ESBLE slightly increased since 2012. Bacteria producing carbapenemase were isolated since 2014. SARM was found high but evolution or resistance seems to be stable during these four-year period. Collecting daily data from clinical settings throughout the country is critical to assess the impact of antimicrobial drug used with Cambodian patients. An implementation of a research study is undertaken to characterize the dissemination of E.coli clones and determine plasmid expression of stains producing ESBL and carbapenemase in Cambodia.