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Tick identification training using molecular methods

Ticks are vectors for many pathogens, making accurate identification crucial for assessing disease risks. In the context of tick surveys, molecular identification is a powerful tool for accurate identification of tick species. From 7-16 August 2023, Dr. Antsa RAKOTONIRINA, PhD from the Institut Pasteur du Cambodge (IPC), provided trainings on tick species identification using COI PCR and MALDI-TOF mass spectrometry. Personnel from IPC, General Directorate of Animal Health and Production (GDAHP), and the Royal University of Agriculture (RUA) participated in this training in Phnom Penh.


The PCR training was held at the GDAHP/NAHPRI molecular laboratory, and the MALDI-TOF training was held at IPC. The trainings consist of both theoretical and practical activities, ranging from classroom lectures to wet lab activities such as DNA extraction, PCR, and gel electrophoresis. These techniques have broad applications in various fields, and the participants will utilize them in their workplace.