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Malaria Research

The research we conduct at the Malaria Research Unit is directed toward a main objective: achieving elimination of this disease from Cambodia. While amazing progress has been done for Plasmodium falciparum, the situation is strikingly different for Plasmodium vivax that requires dedicated intervention, and therefore research, to be eliminated. Our work combines mechanistic questions on the basic biology of host-parasite interactions with very operational research projects in order to develop and implement approaches for the control and elimination of malaria.


Malaria Research

The research we conduct at the Malaria Research Unit is directed toward a main objective: achieving elimination of this disease from Cambodia. While amazing progress has been done for Plasmodium falciparum, the situation is strikingly different for Plasmodium vivax that requires dedicated intervention, and therefore research, to be eliminated. Our work combines mechanistic questions on the basic biology of host-parasite interactions with very operational research projects in order to develop and implement approaches for the control and elimination of malaria.

Main Fields of Research

Antimalarial drug resistance & chemotherapy of malaria parasites

Drug resistance is a major and historical research focus of our unit. A main aspect is related to P. falciparum clinical resistance to artemisinin-based combination therapy (ACT). We are constantly monitoring the resistance patterns of P. falciparum to make sure treatment works optimally in Cambodia and in other areas of South East Asia and inform health authorities to adapt them if they start failing.  We combine in vitro drug susceptibility testing of clinical isolates as well as analysis of molecular markers of resistance. Leveraging a biobank of several hundred of cryopreserved clinical isolates with various resistance genotypes, our work also includes the evaluation of novel antimalarial candidates and research to understand the mechanisms of resistance.

While more challenging, we also monitor antimalarial drug resistance in P. vivax. Our approaches combine in vivo therapeutic efficacy, pharmacokinetics, parasite genomics and in vitro drug response to antimalarials. Indeed, one of our strength is to have implemented short-term in vitro cultures of P. vivax clinical isolates as continuous in vitro growth is still not possible for this species.

Malaria epidemiology in Cambodia and intervention strategies

Our unit is part of the Asia-Pacific International Centre of Excellence in Malaria Research (Asia-Pacific ICEMR Program Director I. Muller/ L. Robinson) which aims at addressing key challenges to malaria elimination in the Asia-Pacific. Together, we carry out a coordinated set of in-depth studies focusing on the epidemiology, entomology and biology of residual malaria transmission at three sites, spanning the entire Asia-Pacific transmission gradient from moderately and high transmission in Papua New Guinea to low, highly focal transmission in Cambodia. Our epidemiological studies combine molecular epidemiology of parasites collected from symptomatic and asymptomatic cases and identification of changes in population structures, serological analyses to identify individuals with markers of recent exposures and molecular analysis of parasites collected from Anopheles mosquitoes captured in the field

Asymptomatic reservoir of parasite carriers is a major obstacle to malaria elimination. This is especially true for P. vivax that not only can lead to low density blood-stage infections undetected by point-of-care diagnostics, but also establishes completely asymptomatic liver hypnozoite infections. Such hypnozoite carriage is undetectable with all currently available diagnostic tools. Our research aims at addressing the issue of asymptomatic/low-density/silent infections in order to reach this reservoir.

P. vivax transmission and liver-stage

The presence of hypnozoites in P. vivax is one of the major factor contributing to the resilience of this species to elimination efforts. We have implemented in our Unit in vitro cultures of P. vivax liver stage allowing the study of hepatic schizonts and dormant hypnozoites using sporozoites obtained through mosquito feeding on patient-derived infectious blood. This unique platform enable us to evaluate transmission-blocking approaches, anti-liver stage drugs and to study the biology of these elusive parasite forms.

Receptors-ligands interactions involved in P. vivax reticulocyte invasion

The implementation of short-term in vitro cultures of P. vivax enabled us to setup controlled invasion assays of clinical isolates into reticulocytes. Such assay allows us to study receptor-ligand interactions involved in reticulocyte invasion and to obtain an unambiguous phenotype of invasion neutralization by antibodies targeting parasite ligands. This is a major achievement and allows to fill critical gaps currently faced by researchers developing blood-stage vaccine against P. vivax. By studying the response to antibodies targeting parasite antigens of a high number of clinical P. vivax isolates collected in Cambodia and elsewhere, we can also explore the genotypic variability of responses to host immunity.

Understanding the basic biology of P. vivax

Because P. vivax malaria was considered wrongly a benign disease and because of the inherent difficulties in studying this parasite, in particular the absence of continuous in vitro culture, our knowledge on this parasite lags far behind what is known for P. falciparum. Thanks to our unique situation at the bedside of infected patients and to state-of-the-art infrastructure and equipment available, we can perform studies to understand the biology of P. vivax. By applying technologies such as single-cell analyses, automated microscope imaging and in vitro phenotypes using live parasites from clinical isolates, we study the mechanisms underlying P. vivax biology notably at two critical steps of its life cycle, the blood and the liver stage.

The Team

Recent Publications

Comparative transcriptomics reveal differential gene expression among Plasmodium vivax geographical isolates and implications on erythrocyte invasion mechanisms.

Kepple D, Ford CT, Williams J, Abagero B, Li S, Popovici J, Yewhalaw D, Lo E.

PLoS Negl Trop Dis. 2024 Jan 29;18(1):e0011926. doi: 10.1371/journal.pntd.0011926. Online ahead of print.


Comparing malaria risk exposure in rural Cambodia population using GPS tracking and questionnaires.

Pepey A, Souris M, Kim S, Obadia T, Chy S, Ea M, Ouk S, Remoue F, Sovannaroth S, Mueller I, Witkowski B, Vantaux A.

Malar J. 2024 Mar 12;23(1):75. doi: 10.1186/s12936-024-04890-6. PMID: 38475843


Intermittent preventive treatment for forest goers by forest malaria workers: an observational study on a key intervention for malaria elimination in Cambodia

Sophea Iv, Chea Nguon, Phanith Kong, Téphanie Siena, Sreynet Srun, Céline Christiansen-Jucht, Chanvong Kul, Thornleaksmey LornSophy ChyJean PopoviciAmélie VantauxBenoit Witkows, Antoine Berry, Patrice Piola, Claude Flamand.

The Lancet Regional Health – Western Pacific Volume 47, June 2024, 101093 https://doi.org/10.1016/j.lanwpc.2024.101093


In Vitro Antimalarial Activity of Trichothecenes against Liver and Blood Stages of Plasmodium

Parvatkar PT, Maher SP, Zhao Y, Cooper CA, de Castro ST, Péneau J, Vantaux A, Witkowski B, Kyle DE, Manetsch R.

J Nat Prod. 2024 Jan 23. doi: 10.1021/acs.jnatprod.3c01019. Online ahead of print


In-vitro assessment of cutaneous immune responses to aedes mosquito salivary gland extract and dengue virus in Cambodian individuals

David Guerrero, Sokchea Lay, Eakpor Piv, Chansophea Chhin, Sokkeang Leng, Ratana Meng, Kim Eng Mam, Polidy Pean, Amelie Vantaux, Sebastien Boyer, Dorothée Missé, Tineke Cantaert

Oxford Open Immunology, Volume 5, Issue 1, 2024, iqae003. Advance Access Publication Date: 1 April 2024 https://doi.org/10.1093/oxfimm/iqae003


Plasmodium ovale spp dhfr mutations associated with reduced susceptibility to pyrimethamine in sub-Saharan Africa: a retrospective genetic epidemiology and functional study.

Joste V, Coppée R, Bailly J, Rakotoarivony Y, Toko Tchokoteu FG, Achache S, Pradines B, Cottrell G, Ariey F, Khim N, Popovici J, Mita T, Groger M, Ramharter M, Egbo T, Juma DW, Akala H, Houzé S, Clain J; Investigation Study Group.

Lancet Microbe. 2024 May, https://doi.org/10.1016/s2666-5247(24)00054-5 . PMID: 38761813


Plasmodium vivax spleen-dependent protein 1 and its role in extracellular vesicles-mediated intrasplenic infections.

Ayllon-Hermida A, Nicolau-Fernandez M, Larrinaga AM, Aparici-Herraiz I, Tintó-Font E, Llorà-Batlle O, Orban A, Yasnot MF, Graupera M, Esteller M, Popovici J, Cortés A, Del Portillo HA, Fernandez-Becerra C.

Front Cell Infect Microbiol. 2024 May 17;14:1408451. https://doi.org/10.3389/fcimb.2024.1408451 . eCollection 2024.PMID: 38828264


PvDBPII elicits multiple antibody-mediated mechanisms that reduce growth in a Plasmodium vivax challenge trial.

Martinez FJ, White M, Guillotte-Blisnick M, Huon C, Boucharlat A, Agou F, England P, Popovici J, Hou MM, Silk SE, Barrett JR, Nielsen CM, Reimer JM, Mukherjee P, Chauhan VS, Minassian AM, Draper SJ, Chitnis CE.

NPJ Vaccines. 2024 Jan 6;9(1):10. doi: 10.1038/s41541-023-00796-7.


Unraveling the intricacies of host-pathogen interaction through single-cell genomics.

Gioacchino E, Vandelannoote K, Ruberto AA, Popovici J, Cantaert T.

Microbes Infect. 2024 Feb 16:105313. doi: 10.1016/j.micinf.2024.105313.


Using serological diagnostics to characterize remaining high-incidence pockets of malaria in forest-fringe Cambodia.

Grimée M, Tacoli C, Sandfort M, Obadia T, Taylor AR, Vantaux A, Robinson LJ, Lek D, Longley RJ, Mueller I, Popovici J, White MT, Witkowski B.

Malar J. 2024 Feb 15;23(1):49. doi: 10.1186/s12936-024-04859-5.

Previous Publications

Antimalarial drug efficacy and resistance in malaria-endemic countries in HANMAT-PIAM_net countries of the Eastern Mediterranean Region 2016-2020: Clinical and genetic studies.

Adam M, Nahzat S, Kakar Q, Assada M, Witkowski B, Tag Eldin Elshafie A, Abuobaida D, Safi N, Khan MA, Nagi M, Mustafa SA, Kohestani K, Muhammad J, Khim N, Al-Hadi M, Elfaki TM, Habib MN, Khairy AKA, Hamid H, Uddin Z, Amer Y, Hassan AH, Elhag MS, Sediqi AW, Kakar I, Abdul-Ghani R, Amran JGH, Abdallrahim TA, Tamim MS, Aljasari A, Rasmussen C, Azkoul L, Warsame M.

Trop Med Int Health. 2023;28(10):817-29. doi:  10.1111/tmi.13929


Hemisynthetic alkaloids derived from trilobine are antimalarials with sustained activity in multidrug-resistant Plasmodium falciparum.

Nardella F, Dobrescu I, Hassan H, Rodrigues F, Thiberge S, Mancio-Silva L, Tafit A, Jallet C, Cadet-Daniel V, Goussin S, Lorthiois A, Menon Y, Molinier N, Pechalrieu D, Long C, Sautel F, Matondo M, Duchateau M, Médard G, Witkowski B, Scherf A, Halby L, Arimondo PB.

iScience. 2023 Jan 11;26(2):105940. doi: 10.1016/j.isci.2023.105940. eCollection 2023 Feb 17.


Human monoclonal antibodies inhibit invasion of transgenic Plasmodium knowlesi expressing Plasmodium vivax Duffy binding protein.

Quentin D. Watson, Lenore L. Carias, Alyssa Malachin, Karli R. Redinger, Jürgen Bosch, Martino Bardelli, Lea Baldor, Lionel Brice Feufack-Donfack, Jean Popovici, Robert W. Moon, Simon J. Draper, Peter A. Zimmerman & Christopher L. King

Malar J. 2023 Dec 4;22(1):369. doi: 10.1186/s12936-023-04766-1.


Immunogenicity of a Plasmodium vivax vaccine based on the duffy binding protein formulated using adjuvants compatible for use in humans.

Francisco J Martinez, Micheline Guillotte-Blisnick, Christèle Huon, Patrick England, Jean Popovici, Hélène Laude, Laurence Arowas, Marie-Noëlle Ungeheuer, Jenny M Reimer, Darrick Carter, Steve Reed, Paushali Mukherjee, Virander S Chauhan, Chetan E Chitnis

Sci Rep. 2023 Aug 25;13(1):13904. doi: 10.1038/s41598-023-40043-6.


Naturally acquired antibodies to gametocyte antigens are associated with reduced transmission of Plasmodium vivax gametocytes to Anopheles arabiensis mosquitoes

Surafel K Tebeje, Wakweya Chali, Elifaged Hailemeskel, Jordache Ramjith, Abrham Gashaw, Temesgen Ashine, Desalegn Nebret, Endashaw Esayas, Tadele Emiru, Tizita Tsegaye, Karina Teelen, Kjerstin Lanke, Eizo Takashima, Takafumi Tsuboi, Nichole D Salinas, Niraj H Tolia, David Narum, Chris Drakeley, Benoit Witkowski, Amelie Vantaux, Matthijs M Jore, William J R Stone, Ivo S Hansen, Fitsum G Tadesse, Teun Bousema

Front Cell Infect Microbiol. 2023 Jan 16:12:1106369. doi: 10.3389/fcimb.2022.1106369. eCollection 2022


Plasmodium vivax Malaria in Duffy-Positive Patients in Rwanda.

Welmoed van Loon, Rafael Oliveira , Clara Bergmann, Felix Habarugira, Costanza Tacoli, Julia Jäger, Darius Savelsberg, Djibril Mbarushimana, Jules M Ndoli, Augustin Sendegeya, Claude Bayingana, Frank P Mockenhaupt

Am J Trop Med Hyg. 2023 Aug 7:tpmd230143. doi: 10.4269/ajtmh.23-0143. Online ahead of print.


Prevalence and characterization of piperaquine, mefloquine and artemisinin derivatives triple-resistant Plasmodium falciparum in Cambodia.

Mairet-Khedim M, Roesch C, Khim N, Srun S, Bouillon A, Kim S, Ke S, Kauy C, Kloeung N, Eam R, Khean C, Kul C, Chy S, Leang R, Ringwald P, Barale JC, Witkowski B.

J Antimicrob Chemother. 2023;78(2):411-7. doi: 10.1093/jac/dkac403


The spatial signature of Plasmodium vivax and Plasmodium falciparum infections: quantifying the clustering of infections in cross-sectional surveys and cohort studies

Mirco Sandfort, Wuelton Monteiro, Marcus Lacerda , Wang Nguitragool, Jetsumon Sattabongkot, Andreea Waltmann, Henrik Salje, Amélie VantauxBenoit Witkowski, Leanne J Robinson, Ivo Mueller, Michael White

Malaria Journal 2023 Mar 4;22(1):75. DOI: 10.1186/s12936-023-04515-4

Asia-Pacific International Center of Excellence in Malaria Research: Maximizing Impact on Malaria Control Policy and Public Health in Cambodia and Papua New Guinea

Leanne J. Robinson, Moses Laman, Leo Makita, Dysoley Lek, Annie Dori, Rachael Farquhar, Amelie Vantaux, Benoit Witkowski, Stephan Karl, Ivo Mueller.

Am J Trop Med Hyg. 2022;107(4_Suppl):124‑30. DOI: 10.4269/ajtmh.21-1324


Efficacy of three anti-malarial regimens for uncomplicated Plasmodium falciparum malaria in Cambodia, 2009-2011: a randomized controlled trial and brief review

Dysoley Lek, Agus Rachmat, Dustin Harrison, Geoffrey Chin, Suwanna Chaoratanakawee, David Saunders, Didier Menard, William O. Rogers.

Malar J. 2022;21(1):259. DOI: 10.1186/s12936-022-04279-3


Evaluation of HRP2 and pLDH-based rapid diagnostic tests for malaria and prevalence of pfhrp2/3 deletions in Aweil, South Sudan

Emily Lynch, Tomas O. Jensen, Bachir Assao, Menard Chihana, Thadeous Turuho, Dan Nyehangane, John B. Manyok, Harriet Pasquale, Nimol Khim, Benoit Witkowski, Matthew E. Coldiron. Malar J. 2022;21(1):261. DOI: 10.1186/s12936-022-04280-w


Metabolic, Pharmacokinetic, and Activity Profile of the Liver Stage Antimalarial (RC-12)

Yuxiang Dong, Yogesh Sonawane, Steven P. Maher, Anne-Marie Zeeman, Victor Chaumeau, Amélie Vantaux, Caitlin A. Cooper, Francis C. K. Chiu, Eileen Ryan, Jenna McLaren, Gong Chen, Sergio Wittlin, Benoît Witkowski, François Nosten, Kamaraj Sriraghavan, Dennis E. Kyle, Clemens H. M. Kocken, Susan A. Charman, Jonathan L. Vennerstrom.

ACS Omega. 2022;7(14):12401‑11. DOI: 10.1021/acsomega.2c01099


Preclinical characterization and target validation of the antimalarial pantothenamide MMV693183

Laura E. de Vries, Patrick A. M. Jansen, Catalina Barcelo, Justin Munro, Julie M. J. Verhoef, Charisse Flerida A. Pasaje, Kelly Rubiano, Josefine Striepen, Nada Abla, Luuk Berning, Judith M. Bolscher, Claudia Demarta-Gatsi, Rob W. M. Henderson, Tonnie Huijs, Karin M. J. Koolen, Patrick K. Tumwebaze, Tomas Yeo, Anna C. C. Aguiar, Iñigo Angulo-Barturen, Alisje Churchyard, Jake Baum, Benigno Crespo Fernández, Aline Fuchs, Francisco-Javier Gamo, Rafael V. C. Guido, María Belén Jiménez-Diaz, Dhelio B. Pereira, Rosemary Rochford, Camille Roesch, Laura M. Sanz, Graham Trevitt, Benoit Witkowski, Sergio Wittlin, Roland A. Cooper, Philip J. Rosenthal, Robert W. Sauerwein, Joost Schalkwijk, Pedro H. H. Hermkens, Roger V. Bonnert, Brice Campo, David A. Fidock, Manuel Llinás, Jacquin C. Niles, Taco W. A. Kooij, Koen J. Dechering.

Nat Commun. 2022;13(1):2158. DOI: 10.1038/s41467-022-29688-5


Single-cell RNA sequencing of Plasmodium vivax sporozoites reveals stage- and species-specific transcriptomic signatures

Anthony A. Ruberto, Caitlin Bourke, Amélie Vantaux, Steven P. Maher, Aaron Jex, Benoit Witkowski, Georges Snounou, Ivo Mueller.

PLoS Negl Trop Dis. 2022;16(8):e0010633. DOI: 10.1371/journal.pntd.0010633

Anopheles ecology, genetics and malaria transmission in northern Cambodia

Amélie Vantaux, Michelle M. Riehle, Eakpor Piv, Elise J. Farley, Sophy Chy, Saorin Kim, Anneli G. Corbett, Rachel L. Fehrman, Anais Pepey, Karin Eiglmeier, Dysoley Lek, Sovannaroth Siv, Ivo Mueller, Kenneth D. Vernick**, Benoit Witkowski**

Sci Rep. 2021;11(1):6458. DOI: 10.1038/s41598-021-85628-1


Artemisinin-independent inhibitory activity of Artemisia sp. infusions against different Plasmodium stages including relapse-causing hypnozoites

Kutub Ashraf*, Shahin Tajeri*, Christophe-Sébastien Arnold, Nadia Amanzougaghene, Jean-François Franetich, Amélie Vantaux, Valérie Soulard, Mallaury Bordessoulles, Guillaume Cazals, Teun Bousema, Geert-Jan van Gemert, Roger Le Grand, Nathalie Dereuddre-Bosquet, Jean-Christophe Barale, Benoit Witkowski, Georges Snounou, Romain Duval, Cyrille Y. Botté, Dominique Mazier.

Life Science Alliance. Life Science Alliance; 2021;5(3). DOI: 10.26508/lsa.202101237


Characterization of the Tubovesicular Network in Plasmodium vivax Liver Stage Hypnozoites and Schizonts

Kayla Sylvester, Steven P. Maher, Dora Posfai, Michael K. Tran, McKenna C. Crawford, Amélie Vantaux, Benoît Witkowski, Dennis E. Kyle, Emily R. Derbyshire.

Frontiers in Cellular and Infection Microbiology; 2021. DOI: 10.3389/fcimb.2021.687019


Choosing interventions to eliminate forest malaria: preliminary results of two operational research studies inside Cambodian forests

Amber Kunkel, Chea Nguon, Sophea Iv, Srean Chhim, Dom Peov, Phanith Kong, Saorin Kim, Sarun Im, Mark Debackere, Nimol Khim, Jean Popovici, Sreynet Srun, Amélie Vantaux, Jean-Olivier Guintran, Benoit Witkowski, Patrice Piola.

Malar J. 2021;20(1):51. DOI: 10.1186/s12936-020-03572-3


Cross-resistance of the chloroquine-derivative AQ-13 with amodiaquine in Cambodian Plasmodium falciparum isolates

Flore Nardella, Mélissa Mairet-Khedim, Camille Roesch, Steven P Maher, Sopheakvatey Ke, Rithea Leang, Didier Leroy, Benoit Witkowski. Journal of Antimicrobial Chemotherapy. 2021;76(10):25658. DOI: 10.1093/jac/dkab219


Development of weight and age-based dosing of daily primaquine for radical cure of vivax malaria

Walter Robert Taylor, Richard M. Hoglund, Pimnara Peerawaranun, Thuy Nhien Nguyen, Tran Tinh Hien, Arnaud Tarantola, Lorenz von Seidlein, Rupam Tripura, Thomas J. Peto, Arjen M. Dondorp, Jordi Landier, Francois H.Nosten, Frank Smithuis, Koukeo Phommasone, Mayfong Mayxay, Soy Ty Kheang, Chy Say, Kak Neeraj, Leang Rithea, Lek Dysoley, Sim Kheng, Sinoun Muth, Arantxa Roca-Feltrer, Mark Debackere, Rick M. Fairhurst, Ngak Song, Philippe Buchy, Didier Menard, Nicholas J. White, Joel Tarning, Mavuto Mukaka.

Malaria Journal. 2021;20(1):366. DOI: 10.1186/s12936-021-03886-w


Dynamics of G6PD activity in patients receiving weekly primaquine for therapy of Plasmodium vivax malaria

Walter R. J. Taylor, Saorin Kim, Sim Kheng, Sinoun Muth, Pety Tor, Eva Christophel, Mavuto Mukaka, Alexandra Kerleguer, Lucio Luzzatto, J. Kevin Baird**, Didier Menard**

PLOS Neglected Tropical Diseases. Public Library of Science; 2021;15(9):e0009690. DOI: 10.1371/journal.pntd.0009690


Efficacy of dihydroartemisinin/piperaquine in patients with non-complicated Plasmodium falciparum malaria in Yaoundé, Cameroon

Mélissa Mairet-Khedim, Sandrine Nsango, Christelle Ngou, Sandie Menard, Camille Roesch, Nimol Khim, Sreynet Srun, Xavier Iriart, Thomas Lanot, Laure Otam, Francis Abega, Lawrence Ayong, Isabelle Morlais, Peggy Gandia, Benoit Witkowski, Antoine Berry.

Journal of Antimicrobial Chemotherapy. 2021;76(11):303744. DOI: 10.1093/jac/dkab281


Primaquine for Plasmodium vivax radical cure: What we do not know and why it matters

Jean Popovici, Kieran Tebben, Benoit Witkowski, David Serre.

Int J Parasitol Drugs Drug Resist. 2021;15:3642. DOI: 10.1016/j.ijpddr.2020.12.004


Probing the distinct chemosensitivity of Plasmodium vivax liver stage parasites and demonstration of 8-aminoquinoline radical cure activity in vitro

Steven P. Maher*, Amélie Vantaux*, Victor Chaumeau, Adeline C. Y. Chua, Caitlin A. Cooper, Chiara Andolina, Julie Péneau, Mélanie Rouillier, Zaira Rizopoulos, Sivchheng Phal, Eakpor Piv, Chantrea Vong, Sreyvouch Phen, Chansophea Chhin, Baura Tat, Sivkeng Ouk, Bros Doeurk, Saorin Kim, Sangrawee Suriyakan, Praphan Kittiphanakun, Nana Akua Awuku, Amy J. Conway, Rays H. Y. Jiang, Bruce Russell, Pablo Bifani, Brice Campo, François Nosten, Benoît Witkowski, Dennis E. Kyle.

Sci Rep. 2021;11(1):19905. DOI: 10.1038/s41598-021-99152-9


Procainamide-SAHA Fused Inhibitors of hHDAC6 Tackle Multidrug-Resistant Malaria Parasites

Flore Nardella, Ludovic Halby, Irina Dobrescu, Johanna Viluma, Corentin Bon, Aurélie Claes, Véronique Cadet-Daniel, Ambre Tafit, Camille Roesch, Elie Hammam, Diane Erdmann, Melissa Mairet-Khedim, Roger Peronet, Salah Mecheri, Benoit Witkowski, Artur Scherf, Paola B. Arimondo.

J Med Chem. 2021;64(14):1040317. DOI: 10.1021/acs.jmedchem.1c00821

Amplification of Duffy binding protein-encoding gene allows Plasmodium vivax to evade host anti-DBP humoral immunity

Popovici Jean*, Roesch Camille*, Carias, Lenore L., Khim Nimol, Kim Saorin, Vantaux Amelie, Mueller Ivo, Chitnis Chetan E., King, Christopher L., Witkowski Benoit.

Nat Commun. 2020;11(1):953. DOI: 10.1038/s41467-020-14574-9


Clinical and in vitro resistance of Plasmodium falciparum to artesunate-amodiaquine in Cambodia

Mairet-Khedim Melissa, Leang Rithea, Marmai Camille, Khim Nimol, Kim Saorin, Ke Sopheakvatey, Kauy Chhayleang, Kloeung Nimol, Eam Rotha, Chy Sophy, Izac Brigitte, Bouth Denis Mey, Bustos Maria Dorina, Ringwald Pascal, Ariey Frederic, Witkowski Benoit.

Clinical Infectious Diseases. 2020;ciaa628. DOI: 10.1093/cid/ciaa628


DNA Methylation Bisubstrate Inhibitors Are Fast-Acting Drugs Active against Artemisinin-Resistant Plasmodium falciparum Parasites

Nardella Flore, Halby Ludovic, Hammam Elie, Erdmann Diane, Cadet-Daniel Véronique, Peronet Roger, Ménard Didier, Witkowski Benoit, Mecheri Salah, Scherf Artur, Arimondo Paola B. 

ACS Cent Sci. 2020;6(1):16‑21. DOI: 10.1021/acscentsci.9b00874


Forest malaria in Cambodia: the occupational and spatial clustering of Plasmodium vivax and Plasmodium falciparum infection risk in a cross-sectional survey in Mondulkiri province, Cambodia”

Sandfort Mirco, Vantaux Amélie, Kim Saorin, Obadia Thomas, Pepey Anaïs, Gardais Soazic, Khim Nimol, Lek Dysoley, White Michael, Robinson Leanne J., Witkowski Benoit, Mueller Ivo.

Malar J. 2020;19(1):413. DOI: 10.1186/s12936-020-03482-4


Identification of compounds active against quiescent artemisinin-resistant Plasmodium falciparum parasites via the quiescent-stage survival assay (QSA)

Reyser Thibaud, Paloque Lucie, Ouji Manel, Nguyen Michel, Ménard Sandie, Witkowski Benoit, Augereau Jean-Michel, Benoit-Vical Françoise.

J Antimicrob Chemother. 2020;75(10):2826‑34. DOI: 10.1093/jac/dkaa250


Impact of the first-line treatment shift from dihydroartemisinin/piperaquine to artesunate/mefloquine on Plasmodium vivax drug susceptibility in Cambodia

Roesch Camille, Mairet-Khedim Mélissa, Kim Saorin, Lek Dysoley, Popovici Jean, Witkowski Benoit.

Journal of Antimicrobial Chemotherapy. 2020;75(7):1766‑71. DOI: 10.1093/jac/dkaa092


Keep the quality high: the benefits of lot testing for the quality control of malaria rapid diagnostic tests

Incardona Sandra, Bell David, Campillo Ana, Cunningham Jane, Ariey Frederic, Fandeur Thierry, Luchavez Jennifer, Luna Christian Anthony, Ménard Didier, Nhem Sina, Sornillo Johanna Beulah, Witkowski Benoit, Katz Zachary, Dittrich Sabine, Ding Xavier C.

Malar J. 2020;19(1):247. DOI: 10.1186/s12936-020-03324-3


Performance of Ultrasensitive Rapid Diagnostic Tests for Detecting Asymptomatic Plasmodium falciparum

Yeung Shunmay,McGregor David,James Nicola,Kheang Soy Ty,Kim Saorin,Khim Nimol,Ly Po,Sovannaroth Siv,Witkowski Benoit.

The American Journal of Tropical Medicine and Hygiene. 2020;102(2):307‑9. DOI: 10.4269/ajtmh.19-0349


Plasmodium vivax Liver and Blood Stages Recruit the Druggable Host Membrane Channel Aquaporin-3

Posfai Dora, Maher Steven P., Roesch Camille, Vantaux Amélie, Sylvester Kayla, Péneau Julie, Popovici Jean, Kyle Dennis E., Witkowski Benoît, Derbyshire Emily R.

Cell Chem Biol. 2020;27(6):719-727.e5. DOI: 10.1016/j.chembiol.2020.03.009


Pyronaridine-artesunate Efficacy and Safety in Uncomplicated Plasmodium falciparum Malaria in Areas of Artemisinin-resistant Falciparum in Viet Nam (2017–2018)

Quang Bui Phuc, Hong Huynh Quang, Thanh Tran Duong, Thanh Le Dong, Quang Nguyen Thieu, Van Truong Hanh, Khim Nimol, Witkowski Benoit, Cong Tran Dai, Bustos Maria Dorina, Ringwald Pascal, Thi Ta Tinh

Clinical Infectious Diseases. 2020;70(10):2187‑95. DOI: 10.1093/cid/ciz580


Quantification of glucose-6-phosphate dehydrogenase activity by spectrophotometry: A systematic review and meta-analysis

Pfeffer Daniel A., Ley Benedikt, Howes Rosalind E., Adu Patrick, Alam Mohammad Shafiul, Bansil Pooja, Boum Yap, Brito Marcelo, Charoenkwan Pimlak, Clements Archie, Cui Liwang, Deng Zeshuai, Egesie Ochaka Julie, Espino Fe Esperanza, von Fricken Michael E., Hamid Muzamil Mahdi Abdel, He Yongshu, Henriques Gisela, Khan Wasif Ali, Khim Nimol, Kim Saorin, Lacerda Marcus, Lon Chanthap, Mekuria Asrat Hailu, Menard Didier, Monteiro Wuelton, Nosten François, Oo Nwe Nwe, Pal Sampa, Palasuwan Duangdao, Parikh Sunil, Pitaloka Pasaribu Ayodhia, Poespoprodjo Jeanne Rini, Price David J., Roca-Feltrer Arantxa, Roh Michelle E., Saunders David L., Spring Michele D., Sutanto Inge, Ley-Thriemer Kamala, Weppelmann Thomas A., von Seidlein Lorenz, Satyagraha Ari Winasti, Bancone Germana, Domingo Gonzalo J., Price Ric N.

PLoS Med. 2020;17(5):e1003084. DOI: 10.1371/journal.pmed.1003084


Significant Efficacy of a Single Low Dose of Primaquine Compared to Stand-Alone Artemisinin Combination Therapy in Reducing Gametocyte Carriage in Cambodian Patients with Uncomplicated Multidrug-Resistant Plasmodium falciparum Malaria

Vantaux Amélie, Kim Saorin, Piv Eakpor, Chy Sophy, Berne Laura, Khim Nimol, Lek Dysoley, Siv Sovannaroth, Mukaka Mavuto, Taylor Walter R., Ménard Didier.

Antimicrob Agents Chemother. 2020;64(6):e02108-19, /aac/64/6/AAC.02108-19.atom. DOI: 10.1128/AAC.02108-19


Studying Land Cover Changes in a Malaria-Endemic Cambodian District: Considerations and Constraints

Pepey Anaïs, Souris Marc, Vantaux Amélie, Morand Serge, Lek Dysoley, Mueller Ivo, Witkowski Benoit, Herbreteau Vincent.

Remote Sensing. Multidisciplinary Digital Publishing Institute; 2020;12(18):2972. DOI: 10.3390/rs12182972


The enigmatic mechanisms by which Plasmodium vivax infects Duffy-negative individuals

Popovici Jean, Roesch Camille, Rougeron Virginie.

PLoS Pathog. 2020;16(2):e1008258. DOI: 10.1371/journal.ppat.1008258



De novo profiling of RNA viruses in Anopheles malaria vector mosquitoes from forest ecological zones in Senegal and Cambodia

Eugeni Belda, Ferdinand Nanfack-Minkeu, Karin Eiglmeier, Guillaume Carissimo, Inge Holm, Mawlouth Diallo, Diawo Diallo, Amélie Vantaux, Saorin Kim, Igor V. Sharakhov, Kenneth D. Vernick.

BMC Genomics. 2019;20(1):664. DOI: 10.1186/s12864-019-6034-1


Efficacy and Safety of Pyronaridine-Artesunate plus Single-Dose Primaquine for the Treatment of Malaria in Western Cambodia

Rithea Leang, Nimol Khim, Huch Chea, Rekol Huy, Melissa Mairet-Khedim, Denis Mey Bouth, Maria Dorina Bustos, Pascal Ringwald, Benoit Witkowski.

Antimicrobial Agents and Chemotherapy. American Society for Microbiology 63(10):e01273-19. DOI: 10.1128/AAC.01273-19


Efficacy and Safety of Pyronaridine-Artesunate plus Single-Dose Primaquine for Treatment of Uncomplicated Plasmodium falciparum Malaria in Eastern Cambodia

Rithea Leang, Melissa Mairet-Khedim, Huch Chea, Rekol Huy, Nimol Khim, Denis Mey Bouth, Maria Dorina Bustos, Pascal Ringwald, Benoit Witkowski.

Antimicrobial Agents and Chemotherapy. American Society for Microbiology 63(3):e02242-18. DOI: 10.1128/AAC.02242-18


High therapeutic efficacy of artemether–lumefantrine and dihydroartemisinin–piperaquine for the treatment of uncomplicated falciparum malaria in Somalia

Marian Warsame, Abdillahi Mohamed Hassan, Abdikarim Hussein Hassan, Ali Mohamed Jibril, Nimol Khim, Abdulkadir Mohamed Arale, Ahamed Hassan Gomey, Zainab Said Nur, Said Mohamed Osman, Marian Said Mohamed, Ali Abdulrahman, Fahmi Essa Yusuf, Jamal Ghilan Hefzullah Amran, Benoit Witkowski, Pascal Ringwald.

Malar J. 2019;18(1):231. DOI: 10.1186/s12936-019-2864-1


Identification and Characterization of Functional Human Monoclonal Antibodies to <i>Plasmodium vivax</i> Duffy-Binding Protein

Lenore L. Carias, Sebastien Dechavanne, Vanessa C. Nicolete, Sokunthea Sreng, Seila Suon, Chanaki Amaratunga, Rick M. Fairhurst, Celia Dechavanne, Samantha Barnes, Benoit Witkowski, Jean Popovici, Camille Roesch, Edwin Chen, Marcelo U. Ferreira, Niraj H. Tolia, John H. Adams, Christopher L. King.

The Journal of Immunology. American Association of Immunologists; 2019; DOI: 10.4049/jimmunol.1801631


In vitro activity of ferroquine against artemisinin-based combination therapy (ACT)-resistant Plasmodium falciparum isolates from Cambodia

Mélissa Mairet-Khedim, Flore Nardella, Nimol Khim, Saorin Kim, Nimol Kloeung, Sopheakvatey Ke, Chhayleang Kauy, Rotha Eam, Chanra Khean, Alain Pellet, Didier Leboulleux, Rithea Leang, Pascal Ringwald, Jean Christophe Barale, Didier Leroy, Didier Menard, Benoit Witkowski.

Journal of Antimicrobial Chemotherapy. 2019;74(11):3240‑4. DOI: 10.1093/jac/dkz340


Lysyl-tRNA synthetase as a drug target in malaria and cryptosporidiosis

Beatriz Baragaña, Barbara Forte, Ryan Choi, Stephen Nakazawa Hewitt, Juan A. Bueren-Calabuig, João Pedro Pisco, Caroline Peet, David M. Dranow, David A. Robinson, Chimed Jansen, Neil R. Norcross, Sumiti Vinayak, Mark Anderson, Carrie F. Brooks, Caitlin A. Cooper, Sebastian Damerow, Michael Delves, Karen Dowers, James Duffy, Thomas E. Edwards, Irene Hallyburton, Benjamin G. Horst, Matthew A. Hulverson, Liam Ferguson, María Belén Jiménez-Díaz, Rajiv S. Jumani, Donald D. Lorimer, Melissa S. Love, Steven Maher, Holly Matthews, Case W. McNamara, Peter Miller, Sandra O’Neill, Kayode K. Ojo, Maria Osuna-Cabello, Erika Pinto, John Post, Jennifer Riley, Matthias Rottmann, Laura M. Sanz, Paul Scullion, Arvind Sharma, Sharon M. Shepherd, Yoko Shishikura, Frederick R. C. Simeons, Erin E. Stebbins, Laste Stojanovski, Ursula Straschil, Fabio K. Tamaki, Jevgenia Tamjar, Leah S. Torrie, Amélie Vantaux, Benoît Witkowski, Sergio Wittlin, Manickam Yogavel, Fabio Zuccotto, Iñigo Angulo-Barturen, Robert Sinden, Jake Baum, Francisco-Javier Gamo, Pascal Mäser, Dennis E. Kyle, Elizabeth A. Winzeler, Peter J. Myler, Paul G. Wyatt, David Floyd, David Matthews, Amit Sharma, Boris Striepen, Christopher D. Huston, David W. Gray, Alan H. Fairlamb, Andrei V. Pisliakov, Chris Walpole, Kevin D. Read, Wesley C. Van Voorhis, Ian H. Gilbert.

Proc Natl Acad Sci USA. 2019;116(14):7015‑20. DOI: 10.1073/pnas.1814685116


Recrudescence, Reinfection, or Relapse? A More Rigorous Framework to Assess Chloroquine Efficacy for Plasmodium vivax Malaria

Jean Popovici, Lindsey Pierce-Friedrich, Saorin Kim, Sophalai Bin, Vorleak Run, Dysoley Lek, Kim Hor Daryl Hee, Lawrence Lee Soon-U, Matthew V Cannon, David Serre, Didier Menard.

The Journal of Infectious Diseases. 2019;219(2):315‑22. DOI: 10.1093/infdis/jiy484


Structural basis for neutralization of Plasmodium vivax by naturally acquired human antibodies that target DBP

Darya Urusova, Lenore Carias, Yining Huang, Vanessa C. Nicolete, Jean Popovici, Camille Roesch, Nichole D. Salinas, Sebastien Dechavanne, Benoit Witkowski, Marcelo U. Ferreira, John H. Adams, Michael L. Gross, Christopher L. King, Niraj H. Tolia.

Nat Microbiol. Nature Publishing Group; 2019;4(9):1486‑96. DOI: 10.1038/s41564-019-0461-2

A comprehensive model for assessment of liver stage therapies targeting Plasmodium vivax and Plasmodium falciparum

Alison Roth, Steven P. Maher, Amy J. Conway, Ratawan Ubalee, Victor Chaumeau, Chiara Andolina, Stephen A. Kaba, Amélie Vantaux, Malina A. Bakowski, Richard Thomson-Luque, Swamy Rakesh Adapa, Naresh Singh, Samantha J. Barnes, Caitlin A. Cooper, Mélanie Rouillier, Case W. McNamara, Sebastian A. Mikolajczak, Noah Sather, Benoît Witkowski, Brice Campo, Stefan H. I. Kappe, David E. Lanar, François Nosten, Silas Davidson, Rays H. Y. Jiang, Dennis E. Kyle, John H. Adams.

Nat Commun. 2018;9(1):1837. DOI: 10.1038/s41467-018-04221-9


Adapting Reactive Case Detection Strategies for falciparum Malaria in a Low-Transmission Area in Cambodia

Gabriele Rossi, Rafael Van den Bergh, Chea Nguon, Mark Debackere, Lieven Vernaeve, Nimol Khim, Saorin Kim, Didier Menard, Martin De Smet, Jean-Marie Kindermans.

Clinical Infectious Diseases. 2018;66(2):296‑8. DOI: 10.1093/cid/cix781


Asymptomatic Plasmodium vivax infections among Duffy-negative population in Kedougou, Senegal

Makhtar Niang, Rokhaya Sane, Abdourahmane Sow, Bacary D. Sadio, Sophy Chy, Eric Legrand, Ousmane Faye, Mawlouth Diallo, Amadou A. Sall, Didier Menard, Aissatou Toure-Balde.

Tropical Medicine and Health. 2018;46(1):45. DOI: 10.1186/s41182-018-0128-3


Closing in on the Reservoir: Proactive Case Detection in High-Risk Groups as a Strategy to Detect Plasmodium falciparum Asymptomatic Carriers in Cambodia

Gabriele Rossi, Lieven Vernaeve, Rafael Van den Bergh, Chea Nguon, Mark Debackere, Carme Abello Peiri, Vuthea Van, Nimol Khim, Saorin Kim, Rotha Eam, Malen Ken, Chanra Khean, Martin De Smet, Didier Menard, Jean-Marie Kindermans.

Clinical Infectious Diseases. 2018;66(10):1610‑7. DOI: 10.1093/cid/cix1064


Contribution to Malaria Transmission of Symptomatic and Asymptomatic Parasite Carriers in Cambodia

Amélie Vantaux, Reingsey Samreth, Eakpor Piv, Nimol Khim, Saorin Kim, Laura Berne, Sophy Chy, Dysoley Lek, Sovannaroth Siv, Walter R Taylor, Didier Ménard.

The Journal of Infectious Diseases. 2018;217(10):1561‑8. DOI: 10.1093/infdis/jiy060


Emerging Southeast Asian PfCRT mutations confer Plasmodium falciparum resistance to the first-line antimalarial piperaquine

Leila S. Ross, Satish K. Dhingra, Sachel Mok, Tomas Yeo, Kathryn J. Wicht, Krittikorn Kümpornsin, Shannon Takala-Harrison, Benoit Witkowski, Rick M. Fairhurst, Frederic Ariey, Didier Menard, David A. Fidock.

Nat Commun. 2018;9(1):3314. DOI: 10.1038/s41467-018-05652-0


Endoperoxide-based compounds: cross-resistance with artemisinins and selection of a Plasmodium falciparum lineage with a K13 non-synonymous polymorphism

Lucie Paloque, Benoit Witkowski, Joël Lelièvre, Manel Ouji, Tanila Ben Haddou, Frédéric Ariey, Anne Robert, Jean-Michel Augereau, Didier Ménard, Bernard Meunier, Françoise Benoit-Vical.

Journal of Antimicrobial Chemotherapy. 2018;73(2):395‑403. DOI: 10.1093/jac/dkx412


Field assessment of potential sugar feeding stations for disseminating bacteria in a paratransgenic approach to control malaria

Etienne Bilgo, Amélie Vantaux, Antoine Sanon, Seni Ilboudo, Roch K. Dabiré, Marcelo Jacobs-Lorena, Abdoulaye Diabate.

Malar J. 2018;17(1):367. DOI: 10.1186/s12936-018-2516-x


Genetic diversity in two Plasmodium vivax protein ligands for reticulocyte invasion

Camille Roesch, Jean Popovici, Sophalai Bin, Vorleak Run, Saorin Kim, Stéphanie Ramboarina, Emma Rakotomalala, Rado Lalaina Rakotoarison, Tsikiniaina Rasoloharimanana, Zo Andriamanantena, Anuj Kumar, Micheline Guillotte-Blisnick, Christèle Huon, David Serre, Chetan E. Chitnis, Inès Vigan-Womas, Didier Menard.

PLOS Neglected Tropical Diseases. Public Library of Science; 2018;12(10):e0006555. DOI: 10.1371/journal.pntd.0006555


Genomic Analyses Reveal the Common Occurrence and Complexity of Plasmodium vivax Relapses in Cambodia

Popovici Jean, Friedrich Lindsey R., Kim Saorin, Bin Sophalai, Run Vorleak, Lek Dysoley, Cannon Matthew V., Menard Didier, Serre David

mBio, 01/23/2018 ; DOI:10.1128/mBio.01888-17


Identification and characterization of areas of high and low risk for asymptomatic malaria infections at sub-village level in Ratanakiri, Cambodia

Lies Durnez, Myrthe Pareyn, Vanna Mean, Saorin Kim, Nimol Khim, Didier Menard, Marc Coosemans, Tho Sochantha, Vincent Sluydts.

Malaria Journal. 2018;17(1):27. DOI: 10.1186/s12936-017-2169-1


Importance of household-level risk factors in explaining micro-epidemiology of asymptomatic malaria infections in Ratanakiri Province, Cambodia

Melanie Bannister-Tyrrell, Set Srun, Vincent Sluydts, Charlotte Gryseels, Vanna Mean, Saorin Kim, Mao Sokny, Koen Peeters Grietens, Marc Coosemans, Didier Menard, Sochantha Tho, Wim Van Bortel, Lies Durnez.

Sci Rep. 2018;8:11643. DOI: 10.1038/s41598-018-30193-3


Prevalence and risk factors for asymptomatic malaria and genotyping of glucose 6-phosphate (G6PD) deficiencies in a vivax-predominant setting, Lao PDR: implications for sub-national elimination goals

Andrew A. Lover, Emily Dantzer, Bouasy Hongvanthong, Keobouphaphone Chindavongsa, Susie Welty, Tania Reza, Nimol Khim, Didier Menard, Adam Bennett.

Malar J. 2018;17:218. DOI: 10.1186/s12936-018-2367-5


Recrudescence, Reinfection, or Relapse? A More Rigorous Framework to Assess Chloroquine Efficacy for Plasmodium vivax Malaria

Jean Popovici, Lindsey Pierce-Friedrich, Saorin Kim, Sophalai Bin, Vorleak Run, Dysoley Lek, Kim Hor Daryl Hee, Lawrence Lee Soon-U, Matthew V Cannon, David Serre, Didier Menard.

The Journal of Infectious Diseases. 2019;219(2):315‑22. DOI: 10.1093/infdis/jiy484


Targeting a Reticulocyte Binding Protein and Duffy Binding Protein to Inhibit Reticulocyte Invasion by Plasmodium vivax

Sonal Gupta, Shailja Singh, Jean Popovici, Camille Roesch, Ahmed Rushdi Shakri, Micheline Guillotte-Blisnick, Christèle Huon, Didier Menard, Chetan E. Chitnis.

Sci Rep. Nature Publishing Group; 2018;8(1):10511. DOI: 10.1038/s41598-018-28757-4


Therapeutic and Transmission-Blocking ?Efficacy of Dihydroartemisinin/Piperaquine and Chloroquine against Plasmodium vivax Malaria, Cambodia

Jean Popovici, Amelie Vantaux, Lyse Primault, Reingsey Samreth, Eak Por Piv, Sophalai Bin, Saorin Kim, Dysoley Lek, David Serre, Didier Menard.

Emerg Infect Dis. 2018;24(8):1516‑9. DOI: 10.3201/eid2408.170768


Antiplasmodial activities of dyes against Plasmodium falciparum asexual and sexual stages: Contrasted uptakes of triarylmethanes Brilliant green, Green S (E142), and Patent Blue V (E131) by erythrocytes

Louis-Jérôme Leba, Jean Popovici, Yannick Estevez, Stéphane Pelleau, Eric Legrand, Lise Musset, Christophe Duplais.

International Journal for Parasitology: Drugs and Drug Resistance. 2017;7(3):314‑20. DOI: 10.1016/j.ijpddr.2017.07.002


Antimalarial Drug Resistance: A Threat to Malaria Elimination

Menard Didier, Dondorp Arjen.

Cold Spring Harb Perspect Med. 2017;7(7):a025619. DOI: 10.1101/cshperspect.a025619


Characterization of P. vivax blood stage transcriptomes from field isolates reveals similarities among infections and complex gene isoforms

Adam Kim, Jean Popovici, Amélie Vantaux, Reingsey Samreth, Sophalai Bin, Saorin Kim, Camille Roesch, Li Liang, Huw Davies, Philip Felgner, Sócrates Herrera, Myriam Arévalo-Herrera, Didier Ménard, David Serre.

Sci Rep. Nature Publishing Group; 2017;7(1):7761. DOI: 10.1038/s41598-017-07275-9


Correction to: Prevalence of K13 mutation and Day-3 positive parasitaemia in artemisinin-resistant malaria endemic area of Cambodia: a cross-sectional study

Soy Ty Kheang, Siv Sovannaroth, Sovann Ek, Say Chy, Phally Chhun, Sokkieng Mao, Sokomar Nguon, Dy Soley Lek, Didier Menard, Neeraj Kak.

Malaria Journal. 2017;16(1):435. DOI: 10.1186/s12936-017-2073-8


Emergence of Indigenous Artemisinin-Resistant Plasmodium falciparum in Africa

Feng Lu, Richard Culleton, Meihua Zhang, Abhinay Ramaprasad, Lorenz von Seidlein, Huayun Zhou, Guoding Zhu, Jianxia Tang, Yaobao Liu, Weiming Wang, Yuanyuan Cao, Sui Xu, Yaping Gu, Julin Li, Chao Zhang, Qi Gao, Didier Menard, Arnab Pain, Haitao Yang, Qingfeng Zhang, Jun Cao.

N Engl J Med. 2017;376(10):991‑3. DOI: 10.1056/NEJMc1612765


Emergence of Plasmodium falciparum triple mutant in Cambodia

Gabriele Rossi, Martin De Smet, Nimol Khim, Jean-Marie Kindermans, Didier Menard.

Lancet Infect Dis. 2017;17(12):1233. DOI: 10.1016/S1473-3099(17)30635-7


Functional analysis of Plasmodium falciparum subpopulations associated with artemisinin resistance in Cambodia

Ankit Dwivedi, Christelle Reynes, Axel Kuehn, Daniel B. Roche, Nimol Khim, Maxim Hebrard, Sylvain Milanesi, Eric Rivals, Roger Frutos, Didier Menard, Choukri Ben Mamoun, Jacques Colinge, Emmanuel Cornillot.

Malar J. 2017;16(1):493. DOI: 10.1186/s12936-017-2140-1


Modelling primaquine-induced haemolysis in G6PD deficiency

James Watson, Walter RJ Taylor, Didier Menard, Sim Kheng, Nicholas J White.

eLife Sciences Publications, Ltd; 2017;6:e23061. DOI: 10.7554/eLife.23061


No evidence for manipulation of Anopheles gambiae, An. coluzzii and An. arabiensis host preference by Plasmodium falciparum

Phuong L. Nguyen*, Amélie Vantaux*, Domonbabele FdS Hien, Kounbobr R. Dabiré, Bienvenue K. Yameogo, Louis-Clément Gouagna, Didier Fontenille, François Renaud, Frédéric Simard, Carlo Costantini, Fréderic Thomas, Anna Cohuet, Thierry Lefèvre.

Sci Rep. 2017;7:9415. DOI: 10.1038/s41598-017-09821-x


Performance of Rapid Diagnostic Testing in Patients with Suspected Malaria in Cambodia, a Low-Endemicity Country Aiming for Malaria Elimination

Gabriele Rossi, Martin De Smet, Nimol Khim, Jean-Marie Kindermans, Didier Menard.

Clinical Infectious Diseases. 2017;65(10):1769‑70. DOI: 10.1093/cid/cix625


Prevalence of K13 mutation and Day-3 positive parasitaemia in artemisinin-resistant malaria endemic area of Cambodia: a cross-sectional study

Soy Ty Kheang, Siv Sovannaroth, Sovann Ek, Say Chy, Phally Chhun, Sokkieng Mao, Sokomar Nguon, Dy Soley Lek, Didier Menard, Neeraj Kak.

Malar J. 2017;16(1):372. DOI: 10.1186/s12936-017-2024-4


Structural adaptations and mechanism of reflex bleeding in the larvae of the myrmecophilous ladybird Diomus thoracicus

Olivier Roux, Amélie Vantaux, Frédéric Petitclerc, Jérôme Orivel, Alain Dejean, Johan Billen.

Arthropod Structure & Development. 2017;46(4):529‑36. DOI: 10.1016/j.asd.2017.06.002


The effect of host plants on genotype variability in fitness and honeydew composition of Aphis fabae

Sharon Schillewaert, Amélie Vantaux, Wim Van den Ende, Tom Wenseleers.

Insect Science. 2017;24(5):781‑8. DOI: 10.1111/1744-7917.12360


The spread of artemisinin-resistant Plasmodium falciparum in the Greater Mekong subregion: a molecular epidemiology observational study

Mallika Imwong, Kanokon Suwannasin, Chanon Kunasol, Kreepol Sutawong, Mayfong Mayxay, Huy Rekol, Frank M. Smithuis, Tin Maung Hlaing, Kyaw M. Tun, Rob W. van der Pluijm, Rupam Tripura, Olivo Miotto, Didier Menard, Mehul Dhorda, Nicholas P. J. Day, Nicholas J. White, Arjen M. Dondorp.

The Lancet Infectious Diseases. Elsevier; 2017;17(5):491‑7. DOI: 10.1016/S1473-3099(17)30048-8


Treatment Failure of Dihydroartemisinin/Piperaquine for Plasmodium falciparum Malaria, Vietnam

Bui Quang Phuc, Charlotte Rasmussen, Tran Thanh Duong, Le Than Dong, Mai Anh Loi, Didier Ménard, Joel Tarning, Dorina Bustos, Pascal Ringwald, Gawrie Loku Galappaththy, Nguyen Quang Thieu.

Emerging Infectious Diseases, 04/2017 ; DOI:10.3201/eid2304.161872

An optimised age-based dosing regimen for single low-dose primaquine for blocking malaria transmission in Cambodia

Rithea Leang, Naw Htee Khu, Mavuto Mukaka, Mark Debackere, Rupam Tripura, Soy Ty Kheang, Say Chy, Neeraj Kak, Philippe Buchy, Arnaud Tarantola, Didier Menard, Arantxa Roca-Felterer, Rick M. Fairhurst, Sim Kheng, Sinoun Muth, Song Ngak, Arjen M. Dondorp, Nicholas J. White, Walter Robert John Taylor.

BMC Med. 2016;14(1):171. DOI: 10.1186/s12916-016-0701-8


Artemisinin-Resistant Plasmodium falciparum K13 Mutant Alleles, Thailand–Myanmar Border

Mikael Boullé, Benoit Witkowski, Valentine Duru, Kanlaya Sriprawat, Shalini K. Nair, Marina McDew-White, Tim J.C. Anderson, Aung Pyae Phyo, Didier Menard, François Nosten.

Emerg Infect Dis. 2016;22(8):1503‑5. DOI: 10.3201/eid2208.160004


A surrogate marker of piperaquine-resistant Plasmodium falciparum malaria: a phenotype-genotype association study

Benoit Witkowski*, Valentine Duru*, Nimol Khim, Leila S. Ross, Benjamin Saintpierre, Johann Beghain, Sophy Chy, Saorin Kim, Sopheakvatey Ke, Nimol Kloeung, Rotha Eam, Chanra Khean, Malen Ken, Kaknika Loch, Anthony Bouillon, Anais Domergue, Laurence Ma, Christiane Bouchier, Rithea Leang, Rekol Huy, Grégory Nuel, Jean-Christophe Barale, Eric Legrand, Pascal Ringwald, David A. Fidock, Odile Mercereau-Puijalon, Frédéric Ariey, Didier Ménard.

Lancet Infect Dis. 2017;17(2):174‑83. DOI: 10.1016/S1473-3099(16)30415-7


A Worldwide Map of Plasmodium falciparum K13-Propeller Polymorphisms

Didier Ménard, Nimol Khim, Johann Beghain, Ayola A. Adegnika, Mohammad Shafiul-Alam, Olukemi Amodu, Ghulam Rahim-Awab, Céline Barnadas, Antoine Berry, Yap Boum, Maria D. Bustos, Jun Cao, Jun-Hu Chen, Louis Collet, Liwang Cui, Garib-Das Thakur, Alioune Dieye, Djibrine Djallé, Monique A. Dorkenoo, Carole E. Eboumbou-Moukoko, Fe-Esperanza-Caridad J. Espino, Thierry Fandeur, Maria-Fatima Ferreira-da-Cruz, Abebe A. Fola, Hans-Peter Fuehrer, Abdillahi M. Hassan, Socrates Herrera, Bouasy Hongvanthong, Sandrine Houzé, Maman L. Ibrahim, Mohammad Jahirul-Karim, Lubin Jiang, Shigeyuki Kano, Wasif Ali-Khan, Maniphone Khanthavong, Peter G. Kremsner, Marcus Lacerda, Rithea Leang, Mindy Leelawong, Mei Li, Khin Lin, Jean-Baptiste Mazarati, Sandie Ménard, Isabelle Morlais, Hypolite Muhindo-Mavoko, Lise Musset, Kesara Na-Bangchang, Michael Nambozi, Karamoko Niaré, Harald Noedl, Jean-Bosco Ouédraogo, Dylan R. Pillai, Bruno Pradines, Bui Quang-Phuc, Michael Ramharter, Milijaona Randrianarivelojosia, Jetsumon Sattabongkot, Abdiqani Sheikh-Omar, Kigbafori D. Silué, Sodiomon B. Sirima, Colin Sutherland, Din Syafruddin, Rachida Tahar, Lin-Hua Tang, Offianan A. Touré, Patrick Tshibangu-wa-Tshibangu, Inès Vigan-Womas, Marian Warsame, Lyndes Wini, Sedigheh Zakeri, Saorin Kim, Rotha Eam, Laura Berne, Chanra Khean, Sophy Chy, Malen Ken, Kaknika Loch, Lydie Canier, Valentine Duru, Eric Legrand, Jean-Christophe Barale, Barbara Stokes, Judith Straimer, Benoit Witkowski, David A. Fidock, Christophe Rogier, Pascal Ringwald, Frederic Ariey, Odile Mercereau-Puijalon.

The New England journal of medicine, 06/23/2016 ; doi:10.1056/NEJMoa1513137


Complexity of Infection and Genetic Diversity in Cambodian Plasmodium vivax

Lindsey R. Friedrich, Jean Popovici, Saorin Kim, Lek Dysoley, Peter A. Zimmerman, Didier Menard, David Serre.

PLoS Negl Trop Dis. 2016;10(3):e0004526. DOI: 10.1371/journal.pntd.0004526


Efficacy and Safety of Pyronaridine-Artesunate for Treatment of Uncomplicated Plasmodium falciparum Malaria in Western Cambodia

Rithea Leang, Sara E. Canavati, Nimol Khim, Lasse S. Vestergaard, Isabelle Borghini Fuhrer, Saorin Kim, Mey Bouth Denis, Pisal Heng, Bunkea Tol, Rekol Huy, Stephan Duparc, Arjen M. Dondorp, Didier Menard, Pascal Ringwald.

Antimicrob Agents Chemother. 2016;60(7):3884‑90. DOI: 10.1128/AAC.00039-16


Efficacy of topical mosquito repellent (picaridin) plus long-lasting insecticidal nets versus long-lasting insecticidal nets alone for control of malaria: a cluster randomised controlled trial

Vincent Sluydts, Lies Durnez, Somony Heng, Charlotte Gryseels, Lydie Canier, Saorin Kim, Karel Van Roey, Karen Kerkhof, Nimol Khim, Sokny Mao, Sambunny Uk, Siv Sovannaroth, Koen Peeters Grietens, Tho Sochantha, Didier Menard, Marc Coosemans.

Lancet Infect Dis. 2016;16(10):1169‑77. DOI: 10.1016/S1473-3099(16)30148-7


Flavone derivatives: a promising tool in the fight against malaria

Nardella, S. Stiebing, P. Wagner, V. Collot, M. Kaiser, B. Witkowski, D. Menard, M. Schmitt, E. Candolfi, C. Vonthron-Sénécheau.

Planta Medica, 12/2016 ; DOI:10.1055/s-0036-1596768


Geographical patterns of malaria transmission based on serological markers for falciparum and vivax malaria in Ratanakiri, Cambodia

Karen Kerkhof, Vincent Sluydts, Somony Heng, Saorin Kim, Myrthe Pareyn, Laura Willen, Lydie Canier, Siv Sovannaroth, Didier Ménard, Tho Sochantha, Marc Coosemans, Lies Durnez.

Malar J. 2016;15(1):510. DOI: 10.1186/s12936-016-1558-1


Identification and Characterization of Two Novel RNA Viruses from Anopheles gambiae Species Complex Mosquitoes

Guillaume Carissimo, Karin Eiglmeier, Julie Reveillaud, Inge Holm, Mawlouth Diallo, Diawo Diallo, Amélie Vantaux, Saorin Kim, Didier Ménard, Sovannaroth Siv, Eugeni Belda, Emmanuel Bischoff, Christophe Antoniewski, Kenneth D. Vernick.

PLOS ONE. Public Library of Science; 2016;11(5):e0153881. DOI: 10.1371/journal.pone.0153881


Low-grade sulfadoxine–pyrimethamine resistance in Plasmodium falciparum parasites from Lubango, Angola

Elsa P. S. Kaingona-Daniel, Larissa Rodrigues Gomes, Bianca E. Gama, Natália K. Almeida-de-Oliveira, Filomeno Fortes, Didier Ménard, Cláudio Tadeu Daniel-Ribeiro, Maria de Fátima Ferreira-da-Cruz.

Malaria Journal. 2016;15(1):309. DOI: 10.1186/s12936-016-1358-7


National Malaria Prevalence in Cambodia: Microscopy Versus Polymerase Chain Reaction Estimates

Dysoley Lek, Jean Popovici, Frederic Ariey, Seshu Babu Vinjamuri, Sylvia Meek, Jan Bruce, Walter R. J. Taylor, Duong Socheat, Didier Menard, William O. Rogers.

Am J Trop Med Hyg. 2016;95(3):588‑94. DOI: 10.4269/ajtmh.15-0908


Plasmodium copy number variation scan: gene copy numbers evaluation in haploid genomes

Johann Beghain, Anne-Claire Langlois, Eric Legrand, Laura Grange, Nimol Khim, Benoit Witkowski, Valentine Duru, Laurence Ma, Christiane Bouchier, Didier Ménard, Richard E. Paul, Frédéric Ariey.

Malar J. 2016;15:206. DOI: 10.1186/s12936-016-1258-x


Plasmodium falciparum parasite population structure and gene flow associated to anti-malarial drugs resistance in Cambodia

Ankit Dwivedi, Nimol Khim, Christelle Reynes, Patrice Ravel, Laurence Ma, Magali Tichit, Christiane Bourchier, Saorin Kim, Dany Dourng, Chanra Khean, Pheaktra Chim, Sovannaroth Siv, Roger Frutos, Dysoley Lek, Odile Mercereau-Puijalon, Frédéric Ariey, Didier Menard, Emmanuel Cornillot.

Malar J. 2016;15:319. DOI: 10.1186/s12936-016-1370-y


Plasmodium falciparum Resistance to Artemisinin Derivatives and Piperaquine: A Major Challenge for Malaria Elimination in Cambodia

Valentine Duru, Benoit Witkowski, Didier Ménard.

Am J Trop Med Hyg. 2016;95(6):1228‑38. DOI: 10.4269/ajtmh.16-0234


Plasmodium vivax Malaria in Cambodia

Sovannaroth Siv, Arantxa Roca-Feltrer, Seshu Babu Vinjamuri, Denis Mey Bouth, Dysoley Lek, Mohammad Abdur Rashid, Ngau Peng By, Jean Popovici, Rekol Huy, Didier Menard.

Am J Trop Med Hyg. 2016;95(6 Suppl):97‑107. DOI: 10.4269/ajtmh.16-0208


Plasmodium vivax multidrug resistance-1 gene polymorphism in French Guiana

Emilie Faway, Lise Musset, Stéphane Pelleau, Béatrice Volney, Jessica Casteras, Valérie Caro, Didier Menard, Sébastien Briolant, Eric Legrand.

Malar J. 2016;15(1):540. DOI: 10.1186/s12936-016-1595-9


Plasmodium vivax mdr1 genotypes in isolates from successfully cured patients living in endemic and non-endemic Brazilian areas

Larissa Rodrigues Gomes, Natália Ketrin Almeida-de-Oliveira, Aline Rosa de Lavigne, Suelen Rezende Félix de Lima, Anielle de Pina-Costa, Patrícia Brasil, Cláudio Tadeu Daniel-Ribeiro, Didier Ménard, Maria de Fatima Ferreira-da-Cruz.

Malaria Journal. 2016;15(1):96. DOI: 10.1186/s12936-016-1141-9


Reactive case-detection of malaria in Pailin Province, Western Cambodia: lessons from a year-long evaluation in a pre-elimination setting

John Hustedt, Sara E. Canavati, Chandary Rang, Ruth A. Ashton, Nimol Khim, Laura Berne, Saorin Kim, Siv Sovannaroth, Po Ly, Didier Ménard, Jonathan Cox, Sylvia Meek, Arantxa Roca-Feltrer.

Malar J. 2016;15(1):132. DOI: 10.1186/s12936-016-1191-z


Role of K13 Mutations in Artemisinin-Based Combination Therapy

Charlotte Rasmussen, Frédéric Ariey, Rick M. Fairhurst, Pascal Ringwald, Didier Ménard.

Clin Infect Dis. 2016;63(12):1680‑1. DOI: 10.1093/cid/ciw641


Serological markers to measure recent changes in malaria at population level in Cambodia

Karen Kerkhof, Vincent Sluydts, Laura Willen, Saorin Kim, Lydie Canier, Somony Heng, Takafumi Tsuboi, Tho Sochantha, Siv Sovannaroth, Didier Ménard, Marc Coosemans, Lies Durnez.

Malar J. 2016;15(1):529. DOI: 10.1186/s12936-016-1576-z



Publications in journals without Impact Factor in 2016


Multiple and successive treatment failures in a patient infected by Plasmodium falciparum in Cambodia and treated by dihydroartemisinin-piperaquine

Witkowski, N. Khim, S. Kim, A. Domergue, V. Duru, D. Menard.

Bull Soc Pathol Exot. 2016;109(2):87‑90. DOI: 10.1007/s13149-016-0487-4



Plasmodium vivax Malaria in Cambodia : Am J Trop Med Hyg, 2016Dec28Siv S / Roca-Feltrer A / Vinjamuri SB / Bouth DM / Lek D / Rashid MA / By NP / Popovici J / Huy R / Menard D / …….


Flavone derivatives: a promising tool in the fight against malaria : Planta Med, 2016Dec, Nardella F / Stiebing S / Wagner P / Collot V / Kaiser M / Witkowski B / Menard D / Schmitt M / Candolfi E / Vonthron-Sénécheau C


Plasmodium vivax multidrug resistance-1 gene polymorphism in French Guiana : Malar J, 2016Nov8, ,Faway E / Musset L / Pelleau S1 / Volney B / Casteras J1 / Caro V / Menard D / Briolant S / Legrand E


A surrogate marker of piperaquine-resistant Plasmodium falciparum malaria: a phenotype-genotype association study : Lancet Infect Dis, 2016Nov3, Witkowski B / Duru V / Khim N / Ross LS / Saintpierre B / Beghain J / Chy S / Kim S / Ke S / Kloeung N / Eam R / Khean C / Ken M / Loch K / …….


Plasmodium falciparum Resistance to Artemisinin Derivatives and Piperaquine: A Major Challenge for Malaria Elimination in Cambodia : Am J Trop Med Hyg, 2016Dec7

Duru V / Witkowski B / Ménard D

An optimised age-based dosing regimen for single low-dose primaquine for blocking malaria transmission in Cambodia, Leang R / Khu NH / Mukaka M / Debackere M / Tripura R / Kheang ST / Chy S / Kak N / Buchy P / Tarantola A / Menard D / Roca-Felterer A / …….


Serological markers to measure recent changes in malaria at population level in Cambodia, Kerkhof K / Sluydts V / Willen L / Kim S / Canier L / Heng S / Tsuboi T / Sochantha T / Sovannaroth S / Ménard D / Coosemans M / Durnez L / ……


Geographical patterns of malaria transmission based on serological markers for falciparum and vivax malaria in Ratanakiri, Cambodia : Malar J. 2016 Oct 19, Kerkhof K / Sluydts V / Heng S / Kim S / Pareyn M / Willen L / Canier L / Sovannaroth S / Ménard D / Sochantha T / Coosemans M / Durnez L


Efficacy of topical mosquito repellent (picaridin) plus long-lasting insecticidal nets versus long-lasting insecticidal nets alone for control of malaria: a cluster randomised controlled trial : Lancet Infect Dis, 2016Oct, (PDF FORMAT) Vincent Sluydts / PhD / Lies Durnez / PhD / Somony Heng / MD / Charlotte Gryseels / MSc / Lydie Canier / MSc / Saorin Kim / BSc / …….

National Malaria Prevalence in Cambodia: Microscopy Versus Polymerase Chain Reaction Estimates : Am J Trop Med Hyg, 2016Sep7. Lek D / Popovici J / Ariey F / Vinjamuri SB / Meek S / Bruce J / Taylor WR / Socheat D / Menard D / Rogers WO

Artemisinin-Resistant Plasmodium falciparum K13 Mutant Alleles, Thailand-Myanmar Border : Emerg Infect Dis, 2016Aug, Boullé M / Witkowski B / Duru V / Sriprawat K / Nair SK / McDew-White M / Anderson TJ / Phyo AP / Menard D / Nosten F


A Worldwide Map of Plasmodium falciparum K13-Propeller Polymorphisms : N Engl J Med, 2016Jun23, Ménard D / Khim N / Beghain J / Adegnika AA / Shafiul-Alam M / Amodu O / Rahim-Awab G / Barnadas C / Berry A / Boum Y / Bustos MD / Cao J / …….


Plasmodium falciparum parasite population structure and gene flow associated to anti‑malarial drugs resistance in Cambodia : Malar J, 2016Jun14, Ankit Dwivedi / Nimol Khim / Christelle Reynes / Patrice Ravel / Laurence Ma / Magali Tichit / Christiane Bourchier / Saorin Kim / …….


Low-grade sulfadoxine-pyrimethamine resistance in Plasmodium falciparum parasites from Lubango, Angola : Malar J, 2016Jun7, Kaingona-Daniel EP / Gomes LR / Gama BE / Almeida-de-Oliveira NK / Fortes F / Ménard D / Daniel-Ribeiro CT / Ferreira-da-Cruz Mde F


Ape malaria transmission and potential for ape-to-human transfers in Africa : Proc Natl Acad Sci U S A, 2016May10, Makanga B / Yangari P / Rahola N / Rougeron V / Elguero E / Boundenga L / Moukodoum ND / Okouga AP / Arnathau C / Durand P / …….


Multiple and successive treatment failures in a patient infected by Plasmodium falciparum in Cambodia and treated by dihydroartemisinin-piperaquine : Bull Soc Pathol Exot, 2016May, Witkowski B / Khim N / Kim S / Domergue A / Duru V / Menard D


Identification and Characterization of Two Novel RNA Viruses from Anopheles gambiae Species Complex Mosquitoes : PLoS One, 2016May3, Carissimo G / Eiglmeier K / Reveillaud J / Holm I / Diallo M / Diallo D / Vantaux A / Kim S / Ménard D / Siv S / Belda E / Bischoff E /…….


An updated checklist of mosquito species (Diptera: Culicidae) from Madagascar : Parasite 2016 (PDF FORMAT), Michaël Luciano Tantely / Gilbert Le Goff / Sébastien Boyer / Didier Fontenille


Plasmodium copy number variation scan: gene copy numbers evaluation in haploid genomes : Malar J, 2016Apr12, Beghain J / Langlois AC / Legrand E / Grange L / Khim N / Witkowski B / Duru V / Ma L / Bouchier C / Ménard D / Paul RE / Ariey F


Complexity of Infection and Genetic Diversity in Cambodian Plasmodium vivax : PLoS Negl Trop Dis, 2016Mar28 (PDF FORMAT ), Friedrich LR / Popovici J / Kim S / Dysoley L / Zimmerman PA / Menard D / Serre D


Reactive case-detection of malaria in Pailin Province, Western Cambodia: lessons from a year-long evaluation in a pre-elimination setting: Malar J, 2016Mar01, Hustedt J / Canavati SE / Rang C / Ashton RA / Khim N / Berne L / Kim S / Sovannaroth S / Ly P / Ménard D / Cox J / Meek S / Roca-Feltrer A


Efficacy and safety of pyronaridine-artesunate for the treatment of uncomplicated Plasmodium falciparum malaria in western Cambodia, Leang R / Canavati SE / Khim N / Vestergaard LS / Borghini Fuhrer I / Kim S / Denis MB / Heng P / Tol B / Huy R / Duparc S / Dondorp AM / …….

Plasmodium Vivax Mdr1 Genotypes in Isolates from Successfully Cured Patients Living in Endemic and Non-Endemic Brazilian Areas : Malar J, 2016Feb18.  Gomes / L. R. / N. K. Almeida-de-Oliveira / A. R. de Lavigne / S. R. de Lima / A. de Pina-Costa / P. Brasil / C. T. Daniel-Ribeiro / D. Menard / ……



Malaria rapid diagnostic test as point-of-care test: study protocol for evaluating the VIKIA® Malaria Ag Pf/Pan. Saorin Kim, Sina Nhem, Dany Dourng and Didier Ménard. Kim et al. Malaria Journal (2015) 14:114, DOI 10.1186/s12936-015-0633-3, March 14, 2015


K13-Propeller Polymorphisms in Plasmodium Falciparum Isolates from Patients in Mayotte in 2013 and 2014. Torrentino-Madamet, M., L. Collet, J. F. Lepere, N. Benoit, R. Amalvict, D. Menard and B. Pradines. “K13-Propeller Polymorphisms in Plasmodium Falciparum Isolates from Patients in Mayotte in 2013 and 2014.” Antimicrob Agents Chemother 59, no. 12 (2015): 7878-81.


Independent Emergence of Artemisinin Resistance Mutations among Plasmodium Falciparum in Southeast Asia. Takala-Harrison, S., C. G. Jacob, C. Arze, M. P. Cummings, J. C. Silva, A. M. Dondorp, M. M. Fukuda, T. T. Hien, M. Mayxay, H. Noedl, F. Nosten, M. P. Kyaw, N. T. Nhien, M. Imwong, D. Bethell, Y. Se, C. Lon, S. D. Tyner, D. L. Saunders, F. Ariey, O. Mercereau-Puijalon, D. Menard, P. N. Newton, M. Khanthavong, B. Hongvanthong, P. Starzengruber, H. P. Fuehrer, P. Swoboda, W. A. Khan, A. P. Phyo, M. M. Nyunt, M. H. Nyunt, T. S. Brown, M. Adams, C. S. Pepin, J. Bailey, J. C. Tan, M. T. Ferdig, T. G. Clark, O. Miotto, B. MacInnis, D. P. Kwiatkowski, N. J. White, P. Ringwald and C. V. Plowe.  J Infect Dis 211, no. 5 (2015): 670-9.


Drug Resistance. K13-Propeller Mutations Confer Artemisinin Resistance in Plasmodium Falciparum Clinical Isolates. Straimer, J., N. F. Gnadig, B. Witkowski, C. Amaratunga, V. Duru, A. P. Ramadani, M. Dacheux, N. Khim, L. Zhang, S. Lam, P. D. Gregory, F. D. Urnov, O. Mercereau-Puijalon, F. Benoit-Vical, R. M. Fairhurst, D. Menard and D. A. Fidock.  Science 347, no. 6220 (2015): 428-31.


Challenges in Antimalarial Drug Treatment for Vivax Malaria Control. Popovici, J. and D. Menard.  Trends Mol Med 21, no. 12 (2015): 776-88.

Reduced Polymorphism in the Kelch Propeller Domain in Plasmodium Vivax Isolates from Cambodia. Popovici, J., S. Kao, L. Eal, S. Bin, S. Kim and D. Menard.  Antimicrob Agents Chemother 59, no. 1 (2015): 730-3.


Low Frequency of Acute Hepatitis E Virus (Hev) Infections but High Past Hev Exposure in Subjects from Cambodia with Mild Liver Enzyme Elevations, Unexplained Fever or Immunodeficiency Due to Hiv-1 Infection. Nouhin J, Barennes H, Madec Y, Prak S, Hou SV, Kerleguer A, Kim S, Pean P, Rouet F. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology 2015, 71:22-27.


Genetic Diversity of Human Rhinoviruses in Cambodia During a Three-Year Period Reveals Novel Genetic Types. Naughtin, M., R. Sareth, A. C. Sentilhes, S. Vong, M. L. Joffret, E. Cornillot, V. Deubel, F. Delpeyroux, R. Frutos and P. Buchy.  Infect Genet Evol 35,  (2015): 42-9.


Towards Real-Time Monitoring of Artemisinin Resistance. Menard, D. and F. Ariey. Lancet Infect Dis 15, no. 4 (2015): 367-8.


Histone Methyltransferase Inhibitors Are Orally Bioavailable, Fast-Acting Molecules with Activity against Different Species Causing Malaria in Humans. Malmquist, N. A., S. Sundriyal, J. Caron, P. Chen, B. Witkowski, D. Menard, R. Suwanarusk, L. Renia, F. Nosten, M. B. Jimenez-Diaz, I. Angulo-Barturen, M. Santos Martinez, S. Ferrer, L. M. Sanz, F. J. Gamo, S. Wittlin, S. Duffy, V. M. Avery, A. Ruecker, M. J. Delves, R. E. Sinden, M. J. Fuchter and A. Scherf.  Antimicrob Agents Chemother 59, no. 2 (2015): 950-9.


Use of Plasmodium Falciparum Culture-Adapted Field Isolates for in Vitro Exflagellation-Blocking Assay. Leba, L. J., L. Musset, S. Pelleau, Y. Estevez, C. Birer, S. Briolant, B. Witkowski, D. Menard, M. J. Delves, E. Legrand, C. Duplais and J. Popovici.  Malar J 14,  (2015): 234.


Evidence of Plasmodium Falciparum Malaria Multidrug Resistance to Artemisinin and Piperaquine in Western Cambodia: Dihydroartemisinin-Piperaquine Open-Label Multicenter Clinical Assessment. Leang, R., W. R. Taylor, D. M. Bouth, L. Song, J. Tarning, M. C. Char, S. Kim, B. Witkowski, V. Duru, A. Domergue, N. Khim, P. Ringwald and D. Menard.  Antimicrob Agents Chemother 59, no. 8 (2015): 4719-26.


Plasmodium Vivax Diversity and Population Structure across Four Continents. Koepfli, C., P. T. Rodrigues, T. Antao, P. Orjuela-Sanchez, P. Van den Eede, D. Gamboa, N. van Hong, J. Bendezu, A. Erhart, C. Barnadas, A. Ratsimbasoa, D. Menard, C. Severini, M. Menegon, B. Y. Nour, N. Karunaweera, I. Mueller, M. U. Ferreira and I. Felger. PLoS Negl Trop Dis 9, no. 6 (2015): e0003872.


Tolerability and Safety of Weekly Primaquine against Relapse of Plasmodium Vivax in Cambodians with Glucose-6-Phosphate Dehydrogenase Deficiency. Kheng, S., S. Muth, W. R. Taylor, N. Tops, K. Kosal, K. Sothea, P. Souy, S. Kim, C. M. Char, C. Vanna, P. Ly, P. Ringwald, V. Khieu, A. Kerleguer, P. Tor, J. K. Baird, S. Bjorge, D. Menard and E. Christophel. BMC Med 13,  (2015): 203.


Implementation and Application of a Multiplex Assay to Detect Malaria-Specific Antibodies: A Promising Tool for Assessing Malaria Transmission in Southeast Asian Pre-Elimination Areas. Kerkhof, K., L. Canier, S. Kim, S. Heng, T. Sochantha, S. Sovannaroth, I. Vigan-Womas, M. Coosemans, V. Sluydts, D. Menard and L. Durnez.  Malar J 14,  (2015): 338.


Novel Cross-Border Approaches to Optimise Identification of Asymptomatic and Artemisinin-Resistant Plasmodium Infection in Mobile Populations Crossing Cambodian Borders. Edwards, H. M., S. E. Canavati, C. Rang, P. Ly, S. Sovannaroth, L. Canier, N. Khim, D. Menard, R. A. Ashton, S. R. Meek and A. Roca-Feltrer.  PLoS One 10, no. 9 (2015): e0124300.


Plasmodium Falciparum Dihydroartemisinin-Piperaquine Failures in Cambodia Are Associated with Mutant K13 Parasites Presenting High Survival Rates in Novel Piperaquine in Vitro Assays: Retrospective and Prospective Investigations. Duru, V., N. Khim, R. Leang, S. Kim, A. Domergue, N. Kloeung, S. Ke, S. Chy, R. Eam, C. Khean, K. Loch, M. Ken, D. Lek, J. Beghain, F. Ariey, P. J. Guerin, R. Huy, O. Mercereau-Puijalon, B. Witkowski and D. Menard.  BMC Med 13,  (2015): 305.


Malaria Pcr Detection in Cambodian Low-Transmission Settings: Dried Blood Spots Versus Venous Blood Samples. Canier, L., N. Khim, S. Kim, R. Eam, C. Khean, K. Loch, M. Ken, P. Pannus, P. Bosman, J. Stassijns, F. Nackers, S. Alipon, M. C. Char, N. Chea, W. Etienne, M. De Smet, J. M. Kindermans and D. Menard.  Am J Trop Med Hyg 92, no. 3 (2015): 573-7.


Value of Routine Dengue Diagnostic Tests in Urine and Saliva Specimens. Andries, A. C., V. Duong, S. Ly, J. Cappelle, K. S. Kim, P. Lorn Try, S. Ros, S. Ong, R. Huy, P. Horwood, M. Flamand, A. Sakuntabhai, A. Tarantola and P. Buchy.  PLoS Negl Trop Dis 9, no. 9 (2015): e0004100.

Field evaluation of picaridin repellents reveals differences in repellent sensitivity between Southeast Asian vectors of malaria and arboviruses. Van Roey K, Sokny M, Denis L, Van den Broeck N, Heng S, Siv S, Sluydts V, Sochantha T, Coosemans M, Durnez L. PLoS Negl Trop Dis. 2014 Dec 18;8(12):e3326. doi: 10.1371/journal.pntd.0003326. eCollection 2014.


[Towards a better understanding of the molecular mechanisms of P. falciparum resistance to artemisinin]. Khim N, Witkowski B, Duru V, Ariey F, Mercereau-Puijalon O, Ménard D.  Med Sci (Paris). 2014 Aug-Sep;30(8-9):727-9. doi: 10.1051/medsci/20143008004. Epub 2014 Sep 1.


Plasmodium falciparum founder populations in western Cambodia have reduced artemisinin sensitivity in vitro. Amaratunga C, Witkowski B, Dek D, Try V, Khim N, Miotto O, Ménard D, Fairhurst RM. Antimicrob Agents Chemother. 2014 Aug;58(8):4935-7. do: 10.1128/AAC.03055-14. Epub 2014 May 27.


Genetic diversity of VAR2CSA ID1-DBL2Xb in worldwide Plasmodium falciparum populations: impact on vaccine design for placental malaria. Bordbar B, Tuikue Ndam N, Renard E, Jafari-Guemouri S, Tavul L, Jennison C, Gnidehou S, Tahar R, Gamboa D, Bendezu J, Menard D, Barry AE, Deloron P, Sabbagh A. Infect Genet Evol. 2014 Jul;25:81-92. doi: 10.1016/j.meegid.2014.04.010. Epub 2014 Apr 21.


Acute undifferentiated febrile illness in rural Cambodia: a 3-year prospective observational study. Mueller TC, Siv S, Khim N, Kim S, Fleischmann E, Ariey F, Buchy P, Guillard B, González IJ, Christophel EM, Abdur R, von Sonnenburg F, Bell D, Menard D. PLoS One. 2014 Apr 22;9(4):e95868. doi: 10.1371/journal.pone.0095868.


A molecular marker of artemisinin-resistant Plasmodium falciparum malaria. Ariey F, Witkowski B, Amaratunga C, Beghain J, Langlois AC, Khim N, Kim S, Duru V, Bouchier C, Ma L, Lim P, Leang R, Duong S, Sreng S, Suon S, Chuor CM, Bout DM, Ménard S, Rogers WO, Genton B, Fandeur T, Miotto O, Ringwald P, Le Bras J, Berry, Barale JC, Fairhurst RM, Benoit-Vical F, Mercereau-Puijalon O, Ménard D. Nature. 2014 Jan 2;505(7481):50-5. doi: 10.1038/nature12876. Epub 2013 Dec 18.



Whole genome sequencing of field isolates reveals a common duplication of the Duffy binding protein gene in Malagasy Plasmodium vivax strains. Menard D, Chan ER, Benedet C, Ratsimbasoa A, Kim S, Chim P, Do C, Witkowski B, Durand R, Thellier M, Severini C, Legrand E, Musset L, Nour BY, Mercereau-Puijalon O, Serre D, Zimmerman PA. PLoS Negl Trop Dis. 2013 Nov 21;7(11):e2489. doi: 10.1371/journal.pntd.0002489. eCollection 2013.


An innovative tool for moving malaria PCR detection of parasite reservoir into the field. Canier L, Khim N, Kim S, Sluydts V, Heng S, Dourng D, Eam R, Chy S, Khean C, Loch K, Ken M, Lim H, Siv S, Tho S, Masse-Navette P, Gryseels C, Uk S, Van Roey K, Grietens KP, Sokny M, Thavrin B, Chuor CM, Deubel V, Durnez L, Coosemans M, Ménard D. Malar J. 2013 Nov 9;12:405. doi: 10.1186/1475-2875-12-405.


Therapeutic efficacy of fixed dose artesunate-mefloquine for the treatment of acute, uncomplicated Plasmodium falciparum malaria in Kampong Speu, Cambodia. Leang R, Ros S, Duong S, Navaratnam V, Lim P, Ariey F, Kiechel JR, Ménard D, Taylor WR (2013). Malar J. 2013 Sep 23;12(1):343.


Novel phenotypic assays for the detection of artemisinin-resistant Plasmodium falciparummalaria in Cambodia: in-vitro and ex-vivo drug-response studies. Witkowski B, Amaratunga C, Khim N, Sreng S, Chim P, Kim S, Lim P, Mao S, Sopha C, Sam B, Anderson JM, Duong S, Meng Chuor C, Taylor WRJ, Suon S, Mercereau-Puijalon O, Fairhurst M, Menard D (2013).  Lancet Infect Dis. 2013 Sep 10.


Ex vivo susceptibility of Plasmodium falciparum to antimalarial drugs in western, northern, and eastern Cambodia, 2011-2012: association with molecular markers. Lim P, Dek D, Try V, Eastman RT, Chy S, Sreng S, Suon S, Mao S, Sopha C, Sam B, Ashley EA, Miotto O, Dondorp AM, White NJ, Su XZ, Chuor CM, Anderson JM, Amaratunga C, Menard D, Fairhurst RM (2013).  Antimicrob Agents Chemother. 2013 Aug 12.


High-Throughput Analysis of Antimalarial Susceptibility Data by the WorldWide Antimalarial Resistance Network (WWARN) In Vitro Analysis and Reporting Tool. Woodrow CJ, Dahlström S, Cooksey R, Flegg JA, Le Nagard H, Mentré F, Murillo C, Ménard D, Nosten F, Sriprawat K, Musset L, Quashie NB, Lim P, Fairhurst RM, Nsobya SL, Sinou V, Noedl H, Pradines B, Johnson JD, Guerin PJ, Sibley CH, Le Bras J. (2013). Antimicrob Agents Chemother. 2013 Jul;57(7):3121-30.


The Evolutionary History of Plasmodium vivax as Inferred from Mitochondrial Genomes: Parasite Genetic Diversity in the Americas. Taylor JE, Pacheco MA, Bacon DJ, Beg MA, Machado RL, Fairhurst RM, Herrera S, Kim JY, Menard D, Póvoa MM, Villegas L, Mulyanto, Snounou G, Cui L, Zeyrek FY, Escalante AA. (2013). The Evolutionary History of Plasmodium vivax as Inferred from Mitochondrial Genomes: Parasite Genetic Diversity in the Americas. Mol Biol Evol. 2013 Jun 28.


In Silico Screening on the Three-dimensional Model of the Plasmodium vivax SUB1 Protease Leads to the Validation of a Novel Anti-parasite Compound. Bouillon A, Giganti D, Benedet C, Gorgette O, Pêtres S, Crublet E, Girard-Blanc C, Witkowski B, Ménard D, Nilges M, Mercereau-Puijalon O, Stoven V, Barale JC. (2013). J Biol Chem. 2013 Jun 21;288(25):18561-73.


Plasmodium falciparum susceptibility to antimalarial drugs: global data issued from the Pasteur Institutes international network. Ménard D, Ariey F, Mercereau-Puijalon O. Plasmodium falciparum susceptibility to antimalarial drugs: global data issued from the Pasteur Institutes international network. Med Sci (Paris). 2013 Jun-Jul;29(6-7):647-55.


G6PD deficiency inPlasmodium falciparum and Plasmodium vivax malaria-infected Cambodian patients. Khim N, Benedet C, Kim S, Kheng S, Siv S, Leang R, Lek S, Muth S, Chea N, Chuor CM, Duong S, Kerleguer A, Tor P, Chim P, Canier L, Witkowski B, Taylor WR, Ménard D. (2013). G6PD deficiency inPlasmodium falciparum and Plasmodium vivax malaria-infected Cambodian patients. Malar J. 2013 May 28;12(1):171.


Review of key knowledge gaps in glucose-6-phosphate dehydrogenase deficiency detection with regard to the safe clinical deployment of 8-aminoquinoline treatment regimens: a workshop report. Von Seidlein L, Auburn S, Espino F, Shanks D, Cheng Q, McCarthy J, Baird K, Moyes C, Howes R, Ménard D, Bancone G, Winasti-Satyahraha A, Vestergaard LS, Green J, Domingo G, Yeung S, Price R. (2013).  Malar J. 2013 Mar 27;12:112.


Reduced Artemisinin Susceptibility of Plasmodium falciparum Ring Stages in Western Cambodia. Witkowski B , Khim N , Chim P , Kim S , Ke S , Kloeung N , Chy S , Duong S, Leang R, Ringwald P, Dondorp AM, Tripura R, Benoit-Vical F, Berry A, Gorgette O, Ariey F, Barale JC, Mercereau-Puijalon O, Menard D. (2013).   Antimicrob Agents Chemother. 2013 Feb;57(2):914-23.


Efficacy of Dihydroartemisinin-Piperaquine for Treatment of Uncomplicated Plasmodium falciparum and Plasmodium vivax in Cambodia, 2008 to 2010. Leang R, Barrette A, Bouth DM, Menard D , Abdur R, Duong S, Ringwald P. (2013).  Antimicrob Agents Chemother. 2013 Feb;57(2):818-26.


Plasmodium falciparum Na+/H+ Exchanger (pfnhe-1) Genetic Polymorphism in Indian Ocean Malaria-Endemic Areas. Andriantsoanirina V, Khim N , Ratsimbasoa A, Witkowski B , Benedet C , Canier L , Bouchier C, Tichit M, Durand R, Ménard D. (2013). Am J Trop Med Hyg. 2013 Jan;88(1):37-42.




Effect of High-Dose or Split-Dose Artesunate on Parasite Clearance in Artemisinin-Resistant Falciparum Malaria. Das D, Tripura R, Phyo AP, Lwin KM, Tarning J, Lee SJ, Hanpithakpong W, Stepniewska K, Menard D ,Ringwald P, Silamut K, Imwong M, Chotivanich K, Yi P, Day NP, Lindegardh N, Socheat D, Nguon C, White NJ, Nosten F, Dondorp AM. (2013).  Clin Infect Dis. 2012 Dec 21.


Global analysis of Plasmodium falciparum Na+/H+ exchanger (pfnhe-1) allele polymorphism and its usefulness as a marker of in vitro resistance to quinine. Didier Ménard , Valérie Andriantsoanirina, Nimol Khim , Arsène Ratsimbasoa, Benoit Witkowski ,Christophe Benedet , Lydie Canier, Odile Mercereau-Puijalon, Rémy Durand (2012).  International Journal for Parasitology: Drugs and Drug Resistance, Volume 3, December 2012, Pages 8-19.


Mapping the Aetiology of Non-Malarial Febrile Illness in Southeast Asia through a Systematic Review-Terra Incognita Impairing Treatment Policies. Nathalie Acestor, Richard Cooksey, Paul N Newton, Didier Ménard, Philippe J Guerin, Jun Nakagawa, Eva Christophel, Iveth J González, David Bell (2012). PLoS One 7: 9. 09


Performance of “VIKIA Malaria Ag Pf/Pan” (IMACCESS®), a new malaria rapid diagnostic test for detection of symptomatic malaria infections. Monidarin Chou, Saorin Kim , Nimol Khim, Sophy Chy, Sarorn Sum, Dany Dourng, Lydie Canier, Chea Nguon, Didier Menard (2012).  Malar J 11: 1. Aug


Fluoroquinolone and pyrazinamide resistance in multidrug-resistant tuberculosis. Pierre-Audigier C, Surcouf C, Cadet-Daniel V, Namouchi A, Heng S, Murray A, Guillard B, Gicquel B. Int J Tuberc Lung Dis. 2012 Feb;16(2):221-3.


Individual Pvmsp1 variants within polyclonal Plasmodium vivax infections display different propensities for relapse. Jessica T Lin, Jonathan J Juliano, Oksana Kharabora, Rithy Sem, Feng-Chang Lin, Sinuon Muth, Didier Ménard, Chansuda Wongsrichanalai, William O Rogers, Steven R Meshnick (2012).  J Clin Microbiol Dec.


Management of uncomplicated malaria in febrile under five-year-old children by community health workers in Madagascar: reliability of malaria rapid diagnostic tests. Arsene Ratsimbasoa, Harintsoa Ravony, Jeanne-Aimee Vonimpaisomihanta, Rogelin Raherinjafy, Martial Jahevitra, Rabenja Rapelanoro, Jean De Dieu Marie Rakotomanga, Denis Malvy, Pascal Millet, Didier Menard (2012).  Malar J 11: 1. Mar  


Whole Genome Sequencing of Field Isolates Provides Robust Characterization of Genetic Diversity in Plasmodium vivax. Ernest R Chan, Didier Menard , Peter H David, Arsène Ratsimbasoa, Saorin Kim, Pheaktra Chim, Catherine Do, Benoit Witkowski, Odile Mercereau-Puijalon, Peter A Zimmerman, David Serre (2012).  PLoS Negl Trop Dis 6: 9. Sep  


Compliance, Safety, and Effectiveness of Fixed-Dose Artesunate-Amodiaquine for Presumptive Treatment of Non-Severe Malaria in the Context of Home Management of Malaria in Madagascar. Arsène Ratsimbasoa, Harintsoa Ravony, Jeanne-Aimée Vonimpaisomihanta, Rogelin Raherinjafy, Martial Jahevitra, Rabenja Rapelanoro, Jean De Dieu Marie Rakotomanga, Denis Malvy, Pascal Millet, Didier Ménard (2012).  Am J Trop Med Hyg 86: 2. 203-210


Focused Screening and Treatment (FSAT): a PCR-based strategy to detect malaria parasite carriers and contain drug resistant P. falciparum, Pailin, Cambodia. F Hoyer S, Nguon S, Kim S , Habib N, Khim N , Sum S , Christophel EM, Bjorge S, Thomson A, Kheng S, Chea N, Yok S, Top S, Ros S, Sophal U, Thompson MM, Mellor S, Ariey F , Witkowski B , Yeang C, Yeung S, Duong S, Newman RD, Menard D . (2012).  PLoS One. 2012;7(10):e45797.


Performance of the CareStart™ G6PD deficiency screening test, a point-of-care diagnostic for primaquine therapy screening. Kim S, Nguon C, Guillard B, Duong S, Chy S, Sum S, Nhem S, Bouchier C, Tichit M, Christophel E, Taylor WR, Baird JK, Menard D.  PLoS One. 2011;6(12):e28357. doi: 10.1371/journal.pone.0028357. Epub 2011 Dec 2.


Reduced impact of pyrimethamine drug pressure on Plasmodium malariae dihydrofolate reductase gene. Nimol Khim, Saorin Kim, Christiane Bouchier, Magali Tichit, Frédéric Ariey, Thierry Fandeur, Pheaktra Chim, Sopheakvatey Ke, Sarorn Sum, Somnang Man, Arsène Ratsimbasoa, Rémy Durand, Didier Ménard (2011).  Antimicrob Agents Chemother Nov


In vitro susceptibility to pyrimethamine of DHFR I164L single mutant Plasmodium falciparum. Andriantsoanirina V, Durand R, Pradines B, Baret E, Bouchier C, Ratsimbasoa A, Menard D.  Malar J. 2011 Sep 27;10(1):283


Resistance of Plasmodium falciparum to antimalarial drugs: impact on malaria pre-elimination in Madagascar. Andriantsoanirina V, Ménard D, Tuséo L, Ratsimbasoa A, Durand R.  Med Trop . 2011 Jun;71(3):298-304.


The global distribution of the Duffy blood group. Howes RE, Patil AP, Piel FB, Nyaniri OA, Kabaria CW, Gething PW, Zimmerman PA, Barnadas C, Beall CM, Gebremedhin A, Ménard D, Williams TN, Weatherall DJ, Hay SI.  Nat Commun. 2011 Apr;2:266.


Multiple independent introductions of Plasmodium falciparum in South America. Erhan Yalcindag, Eric Elguero, Céline Arnathau, Patrick Durand, Jean Akiana, Timothy J Anderson, Agnes Aubouy, François Balloux, Patrick Besnard, Hervé Bogreau, Pierre Carnevale, Umberto D’Alessandro, Didier Fontenille, Dionicia Gamboa, Thibaut Jombart, Jacques Le Mire, Eric Leroy, Amanda Maestre, Mayfong Mayxay, Didier Ménard, Lise Musset, Paul N Newton, Dieudonné Nkoghé, Oscar Noya, Benjamin Ollomo, Christophe Rogier, Vincent Veron, Albina Wide, Sedigheh Zakeri, Bernard Carme, Eric Legrand, Christine Chevillon, Francisco J Ayala, François Renaud, Franck Prugnolle (2011)  Proc Natl Acad Sci U S A Dec  


Plasmodium knowlesi Infection in Humans, Cambodia, 2007-2010. Nimol Khim , Sovannaroth Siv, Saorin Kim, Tara Mueller, Erna Fleischmann, Balbir Singh, Paul Cliff Simon Divis, Nicolas Steenkeste, Linda Duva l, Christiane Bouchier, Socheat Duong, Frederic Ariey ,Didier Menard (2011).  Emerg Infect Dis 17: 10. 1900-1902 Oct   



Exposing malaria in-host diversity and estimating population diversity by capture-recapture using massively parallel pyrosequencing. Juliano JJ, Porter K, Mwapasa V, Sem R, Rogers WO, Ariey F, Wongsrichanalai C, Read A, Meshnick SR.  Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):20138-43.


Chloroquine clinical failures in P. falciparum malaria are associated with mutant Pfmdr-1, not Pfcrt in Madagascar. Andriantsoanirina V, Ratsimbasoa A, Bouchier C, Tichit M, Jahevitra M, Rabearimanana S, Raherinjafy R, Mercereau-Puijalon O, Durand R, Ménard D.  PLoS One. 2010 Oct 13;5(10):e13281.


Plasmodium vivax and the Duffy antigen: A paradigm revisited. Mercereau-Puijalon O, Ménard D.  Transfus Clin Biol. 2010 Sep;17(3):176-183.


Heritability of the human infectious reservoir of malaria parasites. Lawaly YR, Sakuntabhai A, Marrama L, Konate L, Phimpraphi W, Sokhna C, Tall A, Sarr FD, Peerapittayamongkol C, Louicharoen C, Schneider BS, Levescot A, Talman A, Casademont I, Menard D, Trape JF, Rogier C, Kaewkunwal J, Sura T, Nuchprayoon I, Ariey F, Baril L, Singhasivanon P, Mercereau-Puijalon O, Paul R.  PLoS One. 2010 Jun 29;5(6):e11358.


African apes as reservoirs of Plasmodium falciparum and the origin and diversification of the Laverania subgenus. Duval L, Fourment M, Nerrienet E, Rousset D, Sadeuh SA, Goodman SM, Andriaholinirina NV, Randrianarivelojosia M, Paul RE, Robert V, Ayala FJ, Ariey F.  Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10561-6.


OPV strains circulation in HIV infected infants after National Immunisation Days in Bangui, Central African Republic. Manirakiza A, Picard E, Ngbale R, Menard D, Gouandjika-Vasilache I.  BMC Res Notes. 2010 May 18;3:136.


Global sequence variation in the histidine-rich proteins 2 and 3 of Plasmodium falciparum: implications for the performance of malaria rapid diagnostic tests. Baker J, Ho MF, Pelecanos A, Gatton M, Chen N, Abdullah S, Albertini A, Ariey F, Barnwell J, Bell D, Cunningham J, Djalle D, Echeverry DF, Gamboa D, Hii J, Kyaw MP, Luchavez J, Membi C, Menard D, Murillo C, Nhem S, Ogutu B, Onyor P, Oyibo W, Wang SQ, McCarthy J, Cheng Q.  Malar J. 2010 May 17;9:129.


Decreased in vitro susceptibility of Plasmodium falciparum isolates to artesunate, mefloquine, chloroquine, and quinine in Cambodia from 2001 to 2007. Lim P, Wongsrichanalai C, Chim P, Khim N, Kim S, Chy S, Sem R, Nhem S, Yi P, Duong S, Bouth DM, Genton B, Beck HP, Gobert JG, Rogers WO, Coppee JY, Fandeur T, Mercereau-Puijalon O, Ringwald P, Le Bras J, Ariey F.  Antimicrob Agents Chemother. 2010 May;54(5):2135-42.


Sub-microscopic malaria cases and mixed malaria infection in a remote area of high malaria endemicity in Rattanakiri province, Cambodia: implication for malaria elimination. Steenkeste N, Rogers WO, Okell L, Jeanne I, Incardona S,Duval L, Chy S, Hewitt S, Chou M, Socheat D, Babin FX, Ariey F, Rogier C.  Malar J. 2010 Apr 22;9:108.


Plasmodium vivax clinical malaria is commonly observed in Duffy-negative Malagasy people. Ménard D, Barnadas C, Bouchier C, Henry-Halldin C, Gray LR, Ratsimbasoa A, Thonier V, Carod JF, Domarle O, Colin Y, Bertrand O, Picot J, King CL, Grimberg BT, Mercereau-Puijalon O, Zimmerman PA.  Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):5967-71.


Origin and evolution of sulfadoxine resistant Plasmodium falciparum. Vinayak S, Alam MT, Mixson-Hayden T, McCollum AM, Sem R, Shah NK, Lim P, Muth S, Rogers WO, Fandeur T, Barnwell JW, Escalante AA, Wongsrichanalai C, Ariey F F, Meshnick SR, Udhayakumar V.  PLoS Pathog. 2010 Mar 26;6(3):e1000830.


Origins of the recent emergence of Plasmodium falciparum pyrimethamine resistant alleles into Madagascar. Andriantsoanirina V, Bouchier C, Tichit M, Jahevitra M, Rabearimanana S, Randrianjafy R, Ratsimbasoa A, Mercereau-Puijalon O, Durand R, Ménard D.  Antimicrob Agents Chemother. 2010 Mar 22.


Geographic structuring of the Plasmodium falciparum sarco(endo)plasmic reticulum Ca2+ ATPase (PfSERCA) gene diversity. Jambou R, Martinelli A, Pinto J, Gribaldo S, Legrand E, Niang M, Kim N, Pharath L, Volnay B, Ekala MT, Bouchier C, Fandeur T, Berzosa P, Benito A, Ferreira ID, Ferreira C, Vieira PP, Alecrim MG, Mercereau-Puijalon O, Cravo P.  PLoS One. 2010 Feb 25;5(2):e9424.


Multiple genetic backgrounds of the amplified Plasmodium falciparum multidrug resistance (pfmdr1) gene and selective sweep of 184F mutation in Cambodia. Vinayak S, Alam MT, Sem R, Shah NK, Susanti AI, Lim P, Muth S, Maguire JD, Rogers WO, Fandeur T, Barnwell JW, Escalante AA, Wongsrichanalai C, Ariey F F, Meshnick SR, Udhayakumar V. J Infect Dis. 2010 May 15;201(10):1551-60.


Residual antimalarial concentrations before treatment in patients with malaria from Cambodia: indication of drug pressure. Hodel EM, Genton B, Zanolari B, Mercier T, Duong S, Beck HP, Olliaro P, Decosterd LA, Ariey F. Residual antimalarial concentrations before treatment in patients with malaria from Cambodia: indication of drug pressure. J Infect Dis. 2010 Oct 1;202(7):1088-94.


History and current status of Plasmodium falciparum antimalarial drug resistance in Madagascar. Andriantsoanirina V, Ménard D, Tuseo L, Durand R. Scand J Infect Dis. 2010;42(1):22-32.


Microsatellite variations of Plasmodium falciparum Na+/H+ exchanger (Pfnhe-1) gene are not associated with reduced in vitro susceptibility to quinine in clinical isolates from Africa. Andriantsoanirina V, Ménard D, Rabearimanana S, Hubert V, Bouchier C, Tichit M, LeBras J, Durand R.  Am J Trop Med Hyg. 2010.


Low-technology cooling box for storage of malaria RDTs and other medical supplies in remote areas. Chanthap L, Ariey F F, Socheat D, Tsuyuoka R, Bell D.  Malar J. 2010 Jan 23;9:31.



A microarray-based system for the simultaneous analysis of single nucleotide polymorphisms in human genes involved in the metabolism of anti-malarial drugs. Hodel EM, Ley SD, Qi W, Ariey F F, Genton B, Beck HP.  Malar J. 2009 Dec 9;8:285.


Artemisinin resistance in Plasmodium falciparum malaria. Dondorp AM, Nosten F, Yi P, Das D, Phyo AP, Tarning J, Lwin KM, Ariey F F, Hanpithakpong W, Lee SJ, Ringwald P, Silamut K, Imwong M, Chotivanich K, Lim P, Herdman T, An SS, Yeung S, Singhasivanon P, Day NP, Lindegardh N, Socheat D, White NJ.  N Engl J Med. 2009 Jul 30;361(5):455-67. Erratum in: N Engl J Med. 2009 Oct 22;361(17):1714.


FlexiChip package: an universal microarray with a dedicated analysis software for high-thoughput SNPs detection linked to anti-malarial drug resistance. Steenkeste N , Dillies MA, Khim N , Sismeiro O, Chy S , Lim P , Crameri A, Bouchier C, Mercereau-Puijalon O, Beck HP, Imwong M, Dondorp AM, Socheat D, Rogier C, Coppée JY, Ariey F F.  Malar J. 2009 Oct 15;8:229.


Misclassification of drug failure in Plasmodium falciparum clinical trials in southeast Asia. Juliano JJ, Ariey F F, Sem R, Tangpukdee N, Krudsood S, Olson C, Looareesuwan S, Rogers WO, Wongsrichanalai C, Meshnick SR.  J Infect Dis. 2009 Aug 15;200(4):624-8.


Towards high-throughput molecular detection of Plasmodium: new approaches and molecular markers. Steenkeste N, Incardona S, Chy S, Duval L, Ekala MT, Lim P, Hewitt S, Sochantha T, Socheat D, Rogier C, Mercereau-Puijalon O, Fandeur T, Ariey F F.  Malar J. 2009 Apr 29;8:86.


Chimpanzee malaria parasites related to Plasmodium ovale in Africa. Duval L , Nerrienet E, Rousset D, Sadeuh Mba SA, Houze S, Fourment M, Le Bras J, Robert V, Ariey F F.  PLoS One. 2009;4(5):e5520.


Pfmdr1 copy number and arteminisin derivatives combination therapy failure in falciparum malaria in Cambodia. Lim P, Alker AP, Khim N, Shah NK, Incardona S, Doung S, Yi P, Bouth DM, Bouchier C, Puijalon OM, Meshnick SR, Wongsrichanalai C, Fandeur T, Le Bras J, Ringwald P, Ariey F F.  Malar J. 2009 Jan 12;8:11.


Failure of artesunate-mefloquine combination therapy for uncomplicated Plasmodium falciparum malaria in southern Cambodia. Rogers WO, Sem R, Tero T, Chim P, Lim P, Muth S, Socheat D, Ariey F F, Wongsrichanalai C.  Malar J. 2009 Jan 12;8:10.



Molecular surveillance for multidrug-resistant Plasmodium falciparum, Cambodia. Shah NK, Alker AP, Sem R , Susanti AI, Muth S, Maguire JD, Duong S, Ariey F F, Meshnick SR, Wongsrichanalai C.  Emerg Infect Dis. 2008 Oct;14(10):1637-40.


Attraction between sexes: male-female gametocyte behaviour within a Leucocytozoon toddi (Haemosporida). Barraclough RK, Duval L, Talman AM, Ariey F F, Robert V.  Parasitol Res. 2008 May;102(6):1321-7.



Sequence analysis of Plasmodium falciparum cytochrome b in multiple geographic sites. Ekala MT, Khim N, Legrand E, Randrianarivelojosia M, Jambou R, Fandeur T, Menard D, Assi SB, Henry MC, Rogier C, Bouchier C, Mercereau-Puijalon O.  Malar J. 2007 Dec 17;6:164.


Evaluation of the intra- and inter-specific genetic variability of Plasmodium lactate dehydrogenase. Talman AM, Duval L, Legrand E, Hubert V, Yen S, Bell D, Le Bras J, Ariey F F, Houze S.  Malar J. 2007 Oct 25;6:140.


World Antimalarial Resistance Network (WARN) II: in vitro antimalarial drug susceptibility. Bacon DJ, Jambou R, Fandeur T, Le Bras J, Wongsrichanalai C, Fukuda MM, Ringwald P, Sibley CH, Kyle DE.  Malar J. 2007 Sep 6;6:120.


Pfmdr1 and in vivo resistance to artesunate-mefloquine in falciparum malaria on the Cambodian-Thai border. Alker AP, Lim P, Sem R , Shah NK, Yi P, Bouth DM, Tsuyuoka R, Maguire JD, Fandeur T, Ariey F F, Wongsrichanalai C, Meshnick SR.  Am J Trop Med Hyg. 2007 Apr;76(4):641-7.


Large-scale malaria survey in Cambodia: novel insights on species distribution and risk factors. Incardona S, Vong S, Chiv L, Lim P, Nhem S, Sem R, Khim N, Doung S, Mercereau-Puijalon O,Fandeur T.  Malar J. 2007 Mar 27;6:37.


Rapid dissemination of Plasmodium falciparum drug resistance despite strictly controlled antimalarial use. Noranate N, Durand R, Tall A, Marrama L, Spiegel A, Sokhna C, Pradines B, Cojean S, Guillotte M, Bischoff E, Ekala MT, Bouchier C, Fandeur T , Ariey F F, Patarapotikul J, Le Bras J, Trape JF, Rogier C, Mercereau-Puijalon O. PLoS One. 2007 Jan 3;2(1):e139.



Efficacy of artemether-lumefantrine for the treatment of uncomplicated falciparum malaria in northwest Cambodia. Denis MB, Tsuyuoka R, Lim P, Lindegardh N, Yi P, Top SN, Socheat D, Fandeur T, Annerberg A, Christophel EM, Ringwald P.  Trop Med Int Health. 2006 Dec;11(12):1800-7.


Design and synthesis of hydroxyferroquine derivatives with antimalarial and antiviral activities. Biot C, Daher W, Chavain N, Fandeur T, Khalife J, Dive D, De Clercq E.  J Med Chem. 2006 May 4;49(9):2845-9.


Invasion of Africa by a single pfcrt allele of South East Asian type. Ariey F F, Fandeur T, Durand R, Randrianarivelojosia M, Jambou R, Legrand E, Ekala MT, Bouchier C, Cojean S, Duchemin JB, Robert V, Le Bras J, Mercereau-Puijalon O.  Malar J. 2006 Apr 26;5:34.


Assessment of Plasmodium falciparum resistance to ferroquine (SSR97193) in field isolates and in W2 strain under pressure. Daher W, Biot C, Fandeur T, Jouin H, Pelinski L, Viscogliosi E, Fraisse L, Pradines B, Brocard J, Khalife J, Dive D.  Malar J. 2006 Feb 7;5:11.



Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6. Jambou R, Legrand E, Niang M, Khim N , Lim P , Volney B, Ekala MT, Bouchier C, Esterre P, Fandeur T, Mercereau-Puijalon O.  Lancet. 2005 Dec 3;366(9501):1960-3.


Countrywide survey shows very high prevalence of Plasmodium falciparum multilocus resistance genotypes in Cambodia. Khim N, Bouchier C, Ekala MT, Incardona S, Lim P, Legrand E, Jambou R, Doung S, Puijalon OM,Fandeur T.  Antimicrob Agents Chemother. 2005 Aug;49(8):3147-52.


Large sequence heterogeneity of the small subunit ribosomal RNA gene of Plasmodium ovale in Cambodia. Incardona S, Chy S, Chiv L, Nhem S, Sem R, Hewitt S, Doung S, Mercereau-Puijalon O, Fandeur T. Am J Trop Med Hyg. 2005 Jun;72(6):719-24.


In vitro monitoring of Plasmodium falciparum susceptibility to artesunate, mefloquine, quinine and chloroquine in Cambodia: 2001-2002. Lim P, Chim P, Sem R, Nemh S, Poravuth Y, Lim C, Seila S, Tsuyuoka R, Denis MB, Socheat D, Fandeur T.  Acta Trop. 2005 Jan;93(1):31-40.



Importance of protection of antimalarial combination therapies. Duong S, Lim P, Fandeur T, Tsuyuoka R, Wongsrichanalai C.  Lancet. 2004 Nov 13-19;364(9447):1754-5.


Variations in the sequence and expression of the Plasmodium falciparum chloroquine resistance transporter (Pfcrt) and their relationship to chloroquine resistance in vitro. Durrand V, Berry A, Sem R, Glaziou P, Beaudou J, Fandeur T. Mol Biochem Parasitol. 2004 Aug;136(2):273-85.


The in-vitro antimalarial activity of ferrochloroquine, measured against Cambodian isolates of Plasmodium falciparum. Chim P, Lim P, Sem R, Nhem S, Maciejewski L, Fandeur T.  Ann Trop Med Parasitol. 2004 Jun;98(4):419-24.


Chemoresistance and polymorphism in falciparum malaria in Cambodia. Kim N, Bouchier C, Ekala MT, Mercereau-Puijalon O, Fandeur T.  Med Mal Infect. 2004 Jun;34 Suppl 1:S61-4.



Antimalarial activity of artemisinins: identification of a novel target? Mercereau-Puijalon O, Fandeur T. Antimalarial activity of artemisinins: identification of a novel target? Lancet. 2003 Dec 20;362(9401):2035-6.


Update of malaria, multi-drug resistance and economic development in the Mekong region of Southeast Asia. Socheat D, Denis MB, Fandeur T, Zhang Z, Yang H, Xu J, Zhou X, Phompida S, Phetsouvanh R, Lwin S, Lin K, Win T, Than SW, Htut Y, Prajakwong S, Rojanawatsirivet C, Tipmontree R, Vijaykadga S, Konchom S, Cong le D, Thien NT, Thuan le K, Ringwald P, Schapira A, Christophel E, Palmer K, Arbani PR, Prasittisuk C, Rastogi R, Monti F, Urbani C, Tsuyuoka R, Hoyer S, Otega L, Thimasarn K, Songcharoen S, Meert JP, Gay F, Crissman L, Cho-Min-Naing, Chansuda W, Darasri D, Indaratna K, Singhasivanon P, Chuprapawan S, Looareesuwan S, Supavej S, Kidson C, Baimai V, Yimsamran S, Buchachart K. Mekong malaria. II.  Southeast Asian J Trop Med Public Health. 2003;34 Suppl 4:1-102.


pfcrt polymorphism and chloroquine resistance in Plasmodium falciparum strains isolated in Cambodia. Lim P, Chy S, Ariey F, Incardona S, Chim P, Sem R, Denis MB, Hewitt S, Hoyer S, Socheat D, Merecreau-Puijalon O, Fandeur T.  Antimicrob Agents Chemother. 2003 Jan;47(1):87-94.


Efficacy and safety of dihydroartemisinin-piperaquine (Artekin) in Cambodian children and adults with uncomplicated falciparum malaria. Denis MB, Davis TM, Hewitt S, Incardona S, Nimol K, Fandeur T, Poravuth Y, Lim C, Socheat D.  Clin Infect Dis. 2002 Dec 15;35(12):1469-76.